气候变化领域集成服务门户(CCPortal): Gene family evolution underlies cell-type diversification in the hypothalamus of teleosts

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DOI	10.1038/s41559-021-01580-3
	Gene family evolution underlies cell-type diversification in the hypothalamus of teleosts
	Shafer M.E.R.; Sawh A.N.; Schier A.F.
发表日期	2022
ISSN	2397-334X
起始页码	63
结束页码	76
卷号	6 期号:1
英文摘要	Hundreds of cell types form the vertebrate brain but it is largely unknown how similar cellular repertoires are between or within species or how cell-type diversity evolves. To examine cell-type diversity across and within species, we performed single-cell RNA sequencing of ~130,000 hypothalamic cells from zebrafish (Danio rerio) and surface and cave morphs of Mexican tetra (Astyanax mexicanus). We found that over 75% of cell types were shared between zebrafish and Mexican tetra, which diverged from a common ancestor over 150 million years ago. Shared cell types displayed shifts in paralogue expression that were generated by subfunctionalization after genome duplication. Expression of terminal effector genes, such as neuropeptides, was more conserved than the expression of their associated transcriptional regulators. Species-specific cell types were enriched for the expression of species-specific genes and characterized by the neofunctionalization of expression patterns of members of recently expanded or contracted gene families. Comparisons between surface and cave morphs revealed differences in immune repertoires and transcriptional changes in neuropeptidergic cell types associated with genomic differences. The single-cell atlases presented here are a powerful resource to explore hypothalamic cell types and reveal how gene family evolution and shifts in paralogue expression contribute to cellular diversity. ? 2021, The Author(s), under exclusive licence to Springer Nature Limited.
语种	英语
scopus关键词	animal; cave; Characidae; evolution; genetics; hypothalamus; zebra fish; Animals; Biological Evolution; Caves; Characidae; Hypothalamus; Zebrafish
来源期刊	Nature Ecology & Evolution
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257233
作者单位	Biozentrum, University of Basel, Basel, Switzerland; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, United States; Allen Discovery Center for Cell Lineage Tracing, Seattle, WA, United States; Broad Institute of MIT and Harvard, Cambridge, MA, United States; Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States; Center for Brain Science, Harvard University, Cambridge, MA, United States
推荐引用方式 GB/T 7714	Shafer M.E.R.,Sawh A.N.,Schier A.F.. Gene family evolution underlies cell-type diversification in the hypothalamus of teleosts[J],2022,6(1).
APA	Shafer M.E.R.,Sawh A.N.,&Schier A.F..(2022).Gene family evolution underlies cell-type diversification in the hypothalamus of teleosts.Nature Ecology & Evolution,6(1).
MLA	Shafer M.E.R.,et al."Gene family evolution underlies cell-type diversification in the hypothalamus of teleosts".Nature Ecology & Evolution 6.1(2022).