气候变化领域集成服务门户(CCPortal): Environmental selection and epistasis in an empirical phenotype–environment

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DOI	10.1038/s41559-022-01675-5
	Environmental selection and epistasis in an empirical phenotype–environment–fitness landscape
	Chen J.Z.; Fowler D.M.; Tokuriki N.
发表日期	2022
ISSN	2397-334X
起始页码	427
结束页码	438
卷号	6 期号:4
英文摘要	Fitness landscapes, mappings of genotype/phenotype to their effects on fitness, are invaluable concepts in evolutionary biochemistry. Although widely discussed, measurements of phenotype–fitness landscapes in proteins remain scarce. Here, we quantify all single mutational effects on fitness and phenotype (EC50) of VIM-2 β-lactamase across a 64-fold range of ampicillin concentrations. We then construct a phenotype–fitness landscape that takes variations in environmental selection pressure into account. We found that a simple, empirical landscape accurately models the ~39,000 mutational data points, suggesting that the evolution of VIM-2 can be predicted on the basis of the selection environment. Our landscape provides new quantitative knowledge on the evolution of the β-lactamases and proteins in general, particularly their evolutionary dynamics under subinhibitory antibiotic concentrations, as well as the mechanisms and environmental dependence of non-specific epistasis. ? 2022, The Author(s), under exclusive licence to Springer Nature Limited.
语种	英语
scopus关键词	protein; biological model; epistasis; mutation; phenotype; reproductive fitness; Epistasis, Genetic; Genetic Fitness; Models, Genetic; Mutation; Phenotype; Proteins
来源期刊	Nature Ecology & Evolution
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257104
作者单位	Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada; Department of Genome Sciences, University of Washington, Seattle, WA, United States; Department of Bioengineering, University of Washington, Seattle, WA, United States
推荐引用方式 GB/T 7714	Chen J.Z.,Fowler D.M.,Tokuriki N.. Environmental selection and epistasis in an empirical phenotype–environment–fitness landscape[J],2022,6(4).
APA	Chen J.Z.,Fowler D.M.,&Tokuriki N..(2022).Environmental selection and epistasis in an empirical phenotype–environment–fitness landscape.Nature Ecology & Evolution,6(4).
MLA	Chen J.Z.,et al."Environmental selection and epistasis in an empirical phenotype–environment–fitness landscape".Nature Ecology & Evolution 6.4(2022).