气候变化领域集成服务门户(CCPortal): Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster

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DOI	10.1038/s41559-022-01697-z
	Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster
	Mongelli V.; Lequime S.; Kousathanas A.; Gausson V.; Blanc H.; Nigg J.; Quintana-Murci L.; Elena S.F.; Saleh M.-C.
发表日期	2022
ISSN	2397-334X
起始页码	565
结束页码	578
卷号	6 期号:5
英文摘要	Host–pathogen interactions impose recurrent selective pressures that lead to constant adaptation and counter-adaptation in both competing species. Here, we sought to study this evolutionary arms-race and assessed the impact of the innate immune system on viral population diversity and evolution, using Drosophila melanogaster as model host and its natural pathogen Drosophila C virus (DCV). We isogenized eight fly genotypes generating animals defective for RNAi, Imd and Toll innate immune pathways as well as pathogen-sensing and gut renewal pathways. Wild-type or mutant flies were then orally infected with DCV and the virus was serially passaged ten times via reinfection in naive flies. Viral population diversity was studied after each viral passage by high-throughput sequencing and infection phenotypes were assessed at the beginning and at the end of the evolution experiment. We found that the absence of any of the various immune pathways studied increased viral genetic diversity while attenuating virulence. Strikingly, these effects were observed in a range of host factors described as having mainly antiviral or antibacterial functions. Together, our results indicate that the innate immune system as a whole and not specific antiviral defence pathways in isolation, generally constrains viral diversity and evolution. ? 2022, The Author(s), under exclusive licence to Springer Nature Limited.
语种	英语
来源期刊	Nature Ecology & Evolution
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257085
作者单位	Viruses and RNA Interference Unit, Institut Pasteur, CNRS, Paris, France; Cluster of Microbial Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, Netherlands; Human Evolutionary Genetic Unit, Institut Pasteur, CNRS, Paris, France; Human Genomics and Evolution, Collège de France, Paris, France; Instituto de Biología Integrativa de Sistemas (CSIC—Universitat de València), València, Spain; The Santa Fe Institute, Santa Fe, NM, United States
推荐引用方式 GB/T 7714	Mongelli V.,Lequime S.,Kousathanas A.,et al. Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster[J],2022,6(5).
APA	Mongelli V..,Lequime S..,Kousathanas A..,Gausson V..,Blanc H..,...&Saleh M.-C..(2022).Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster.Nature Ecology & Evolution,6(5).
MLA	Mongelli V.,et al."Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster".Nature Ecology & Evolution 6.5(2022).