气候变化领域集成服务门户(CCPortal): Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection

CCPortal

DOI	10.1038/s41559-022-01705-2
	Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection
	Yao Y.; Maddamsetti R.; Weiss A.; Ha Y.; Wang T.; Wang S.; You L.
发表日期	2022
ISSN	2397-334X
起始页码	555
结束页码	564
卷号	6 期号:5
英文摘要	The spread of genes encoding antibiotic resistance is often mediated by horizontal gene transfer (HGT). Many of these genes are associated with transposons, a type of mobile genetic element that can translocate between the chromosome and plasmids. It is widely accepted that the translocation of antibiotic resistance genes onto plasmids potentiates their spread by HGT. However, it is unclear how this process is modulated by environmental factors, especially antibiotic treatment. To address this issue, we asked whether antibiotic exposure would select for the transposition of resistance genes from chromosomes onto plasmids and, if so, whether antibiotic concentration could tune the distribution of resistance genes between chromosomes and plasmids. We addressed these questions by analysing the transposition dynamics of synthetic and natural transposons that encode resistance to different antibiotics. We found that stronger antibiotic selection leads to a higher fraction of cells carrying the resistance on plasmids because the increased copy number of resistance genes on multicopy plasmids leads to higher expression of those genes and thus higher cell survival when facing antibiotic selection. Once they have transposed to plasmids, antibiotic resistance genes are primed for rapid spread by HGT. Our results provide quantitative evidence for a mechanism by which antibiotic selection accelerates the spread of antibiotic resistance in microbial communities. ? 2022, The Author(s), under exclusive licence to Springer Nature Limited.
语种	英语
scopus关键词	antiinfective agent; antibiotic resistance; genetics; horizontal gene transfer; interspersed repeat; plasmid; Anti-Bacterial Agents; Drug Resistance, Microbial; Gene Transfer, Horizontal; Interspersed Repetitive Sequences; Plasmids
来源期刊	Nature Ecology & Evolution
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257072
作者单位	Department of Biomedical Engineering, Duke University, Durham, NC, United States; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, United States; Center for Genomic and Computational Biology, Duke University, Durham, NC, United States
推荐引用方式 GB/T 7714	Yao Y.,Maddamsetti R.,Weiss A.,et al. Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection[J],2022,6(5).
APA	Yao Y..,Maddamsetti R..,Weiss A..,Ha Y..,Wang T..,...&You L..(2022).Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection.Nature Ecology & Evolution,6(5).
MLA	Yao Y.,et al."Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection".Nature Ecology & Evolution 6.5(2022).