气候变化领域集成服务门户(CCPortal): Overexpression of Twist1 in vascular endothelial cells promotes pathological retinal angiogenesis in mice

CCPortal

DOI	10.24272/j.issn.2095-8137.2021.281
	Overexpression of Twist1 in vascular endothelial cells promotes pathological retinal angiogenesis in mice
	Zhang, Lin; Zhang, Shan-Shan; Wang, Kai-Fang; Li, Yi-Hui; Xu, Hui-Juan; Sun, Kuan-Xiang; Ma, Shi; Leng, Hong-Mei; Chen, Si-Zhu; Jia, Wen-Jing; Zhu, Xian-Jun; Li, Jie
通讯作者	Li, J (通讯作者)，Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Dept Ophthalmol, Chengdu 610072, Sichuan, Peoples R China. ; Li, J (通讯作者)，Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China. ; Li, J (通讯作者)，Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Dept Lab Med, Chengdu 610072, Sichuan, Peoples R China. ; Li, J (通讯作者)，Sichuan Acad Med Sci, Res Unit Blindness Prevent Chinese Acad Med Sci 2, Chengdu 610072, Sichuan, Peoples R China.
发表日期	2022
ISSN	2095-8137
起始页码	64
结束页码	74
卷号	43 期号:1
英文摘要	Retinal angiogenesis is a critical process for normal retinal function. However, uncontrolled angiogenesis can lead to pathological neovascularization (NV), which is closely related to most irreversible blindness-causing retinal diseases. Understanding the molecular basis behind pathological NV is important for the treatment of related diseases. Twist-related protein 1 (TWIST1) is a well-known transcription factor and principal inducer of epithel-ialmesenchymal transition (EMT) in many human cancers. Our previous study showed that Twist1 expression is elevated in pathological retinal NV. To date, however, the role of TWIST1 in retinal pathological angiogenesis remains to be elucidated. To study the role of TWIST1 in pathological retinal NV and identify specific molecular targets for antagonizing pathological NV, we generated an inducible vascular endothelial cell (EC)-specific Twist1 transgenic mouse model (Tg-Twist1(iEC+)). Whole-mount retinas from Tg-Twist(1iEC+) mice showed retarded vascular progression and increased vascular density in the front end of the growing retinal vasculature, as well as aneurysm-like pathological retinal NV. Furthermore, overexpression of Twist1 in the ECs promoted cell proliferation but disturbed cell polarity, thus leading to uncontrolled retinal angiogenesis. TWIST1 promoted pathological NV by activating the Wnt/beta-catenin signaling pathway and inducing the expression of NV formation-related genes, thereby acting as a 'valve' in the regulation of pathological angiogenesis. This study identified the critical role of TWIST1 in retinal pathological NV, thus providing a potential therapeutic target for pathological NV.
关键词	MORPHOGENESIS RETINOPATHY METASTASIS ACTIVATION GENE
英文关键词	Pathological angiogenesis; TWIST1; Molecular markers; Mouse model; Retinal neovascularization
语种	英语
WOS研究方向	Zoology
WOS类目	Zoology
WOS记录号	WOS:000740658200009
来源期刊	ZOOLOGICAL RESEARCH
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/255172
作者单位	[Zhang, Lin; Xu, Hui-Juan; Leng, Hong-Mei; Chen, Si-Zhu; Li, Jie] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Dept Ophthalmol, Chengdu 610072, Sichuan, Peoples R China; [Zhang, Lin; Zhang, Shan-Shan; Wang, Kai-Fang; Li, Yi-Hui; Xu, Hui-Juan; Sun, Kuan-Xiang; Ma, Shi; Zhu, Xian-Jun; Li, Jie] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China; [Zhang, Lin; Zhang, Shan-Shan; Wang, Kai-Fang; Li, Yi-Hui; Xu, Hui-Juan; Sun, Kuan-Xiang; Ma, Shi; Zhu, Xian-Jun; Li, Jie] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sch Med, Dept Lab Med, Chengdu 610072, Sichuan, Peoples R China; [Zhang, Lin; Zhang, Shan-Shan; Wang, Kai-Fang; Li, Yi-Hui; Xu, Hui-Juan; Sun, Kuan-Xiang; Ma, Shi; Zhu, Xian-Jun; Li, Jie] Sichuan Acad Med Sci, Res Unit Blindness Prevent Chinese Acad Med Sci 2, Chengdu 610072, Sichuan, Peoples R China; [Zhang, Lin; Jia, Wen-Jing; Zhu, Xian-Jun] Chinese Acad Sci, North...
推荐引用方式 GB/T 7714	Zhang, Lin,Zhang, Shan-Shan,Wang, Kai-Fang,et al. Overexpression of Twist1 in vascular endothelial cells promotes pathological retinal angiogenesis in mice[J]. 中国科学院西北生态环境资源研究院,2022,43(1).
APA	Zhang, Lin.,Zhang, Shan-Shan.,Wang, Kai-Fang.,Li, Yi-Hui.,Xu, Hui-Juan.,...&Li, Jie.(2022).Overexpression of Twist1 in vascular endothelial cells promotes pathological retinal angiogenesis in mice.ZOOLOGICAL RESEARCH,43(1).
MLA	Zhang, Lin,et al."Overexpression of Twist1 in vascular endothelial cells promotes pathological retinal angiogenesis in mice".ZOOLOGICAL RESEARCH 43.1(2022).