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DOI10.1016/j.foodchem.2021.129415
Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling
Xu, Jingke; Dou, Leina; Liu, Sijie; Su, Lihong; Yin, Xuechi; Ren, Jing; Hu, Huilan; Zhang, Daohong; Sun, Jing; Wang, Zhanhui; Wang, Jianlong
通讯作者Wang, JL (通讯作者),Northwest A&F Univ, Coll Food Sci & Engn, 22Xinong Rd, Yangling 712100, Shanxi, Peoples R China. ; Wang, ZH (通讯作者),China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing 100193, Peoples R China. ; Wang, ZH (通讯作者),Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China.
发表日期2021
ISSN0308-8146
EISSN1873-7072
卷号352
英文摘要Furazolidone (FZD) and its metabolite called 3-amino-2-oxazolidinone (AOZ) would induce carcinogenic and mutagenic effects to human. In this work, to develop a novel, stable, and simple point of care testing (POCT) with a potential to social applied for FZD detection, we utilized the aspect of protein staining of coomassie brilliant blue (CBB) to exploit a new CBB-LFIA strategy free of NPs. Only one mixing step is needed during the probe manufacturing process, which requires just 2 h and is a great time saving strategy compared with other methods (requiring 4?33 h for probe preparation). Besides, the cost of CBB-LFIA is 300 times lesser than other LFIA with respect to obtaining the label. The developed CBB-LFIA was successfully applied to detect AOZ with a detection limit of 2 ng mL-1, without any influence from other potential interfering compounds. The proposed CBB-LFIA exhibited prominent practical application, and possesses considerable utilization potential in the related field.
英文关键词Lateral flow immunoassay; Point-of-care testing; Coomassie brilliant blue; Furazolidone detection
语种英语
WOS研究方向Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS类目Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号WOS:000636067600001
来源期刊FOOD CHEMISTRY
来源机构中国科学院西北生态环境资源研究院
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/255018
作者单位[Xu, Jingke; Liu, Sijie; Su, Lihong; Yin, Xuechi; Ren, Jing; Hu, Huilan; Zhang, Daohong; Wang, Jianlong] Northwest A&F Univ, Coll Food Sci & Engn, 22Xinong Rd, Yangling 712100, Shanxi, Peoples R China; [Dou, Leina; Wang, Zhanhui] China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing 100193, Peoples R China; [Dou, Leina; Wang, Zhanhui] Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China; [Sun, Jing] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, 23Xinning Rd, Xining 810008, Qinghai, Peoples R China
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Xu, Jingke,Dou, Leina,Liu, Sijie,et al. Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling[J]. 中国科学院西北生态环境资源研究院,2021,352.
APA Xu, Jingke.,Dou, Leina.,Liu, Sijie.,Su, Lihong.,Yin, Xuechi.,...&Wang, Jianlong.(2021).Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling.FOOD CHEMISTRY,352.
MLA Xu, Jingke,et al."Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling".FOOD CHEMISTRY 352(2021).
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