气候变化领域集成服务门户(CCPortal): Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae)

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DOI	10.1016/j.jplph.2021.153575
	Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae)
	Liu, Yu Ping; Zhang, Yu; Liu, Feng; Liu, Tao; Chen, Jin Yuan; Fu, Gui; Zheng, Chang Yuan; Su, Dan Dan; Wang, Ya Nan; Zhou, Hua Kun; Su, Xu; Harris, A. J.; Wang, Xiu Mei
通讯作者	Su, X (通讯作者)，Qinghai Normal Univ, Sch Life Sci, Xining 810008, Peoples R China. ; Harris, AJ (通讯作者)，Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Peoples R China.
发表日期	2022
ISSN	0176-1617
EISSN	1618-1328
卷号	268
英文摘要	Psammochloa villosa is a desert plant growing in Northwest China with considerable resistance to abiotic stress, including drought, cold, and salt. To facilitate future studies of stress resistance in Psammochloa villosa, we sought to establish a suite of reference (or housekeeping) genes for utilization within future gene expression studies. Specifically, we selected nine candidate genes based on prior studies and new transcriptomic data for P. villosa, and we evaluated their expression stability in three different tissues of P. villosa under different treatments simulating abiotic stress conditions using four different bioinformatics assessments. Our results showed that TIP41 (TIP41-like family protein) was the most stable reference gene in drought- and salt-stressed leaves and saltstressed stems, ELF-1 alpha (elongation factor 1-alpha) was the most stable in cold-stressed leaves and drought- and saltstressed roots, ACT (actin) was the most stable in drought-stressed stems, TUA (alpha-tubulin) was the most stable in cold-stressed stems, and 18S rRNA (18S ribosomal RNA) was the most stable in cold-stressed roots. Additionally, we tested the utility of these candidate reference genes to detect the expression pattern of P5CS (Delta 1pyrroline-5-carboxylate synthetase), which is a drought-related gene. This study is the first report on selecting and validating reference genes of P. villosa under various stress conditions and will benefit future investigations of the genomic mechanisms of stress resistance in this ecologically important species.
关键词	QRT-PCR SELECTION NORMALIZATION VALIDATION EXPRESSION OVEREXPRESSION QUANTIFICATION DROUGHT
英文关键词	Desert plants; Qinghai-Tibetan Plateau; RefFinder; Stipeae; Stress treatment; Transcriptome
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000744260900005
来源期刊	JOURNAL OF PLANT PHYSIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254990
作者单位	[Liu, Yu Ping; Zhang, Yu; Liu, Feng; Chen, Jin Yuan; Zheng, Chang Yuan; Su, Dan Dan; Wang, Ya Nan; Su, Xu; Wang, Xiu Mei] Qinghai Normal Univ, Sch Life Sci, Xining 810008, Peoples R China; [Liu, Yu Ping; Su, Xu] Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Xining 810016, Peoples R China; [Harris, A. J.] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Peoples R China; [Liu, Yu Ping; Su, Xu] Qinghai Normal Univ, Key Lab Med Anim & Plant Resources Qinghai Tibet, Xining 810008, Peoples R China; [Liu, Tao; Fu, Gui] Qinghai Normal Univ, Sch Geog, Xining 810008, Peoples R China; [Liu, Tao; Su, Xu] Qinghai Normal Univ, Key Lab Land Surface Proc & Ecol Conservat Qingha, Minist Educ, Xining 810008, Peoples R China; [Zhou, Hua Kun] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Cold Reg Restorat Ecol Qinghai Prov, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yu Ping,Zhang, Yu,Liu, Feng,et al. Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae)[J]. 中国科学院西北生态环境资源研究院,2022,268.
APA	Liu, Yu Ping.,Zhang, Yu.,Liu, Feng.,Liu, Tao.,Chen, Jin Yuan.,...&Wang, Xiu Mei.(2022).Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae).JOURNAL OF PLANT PHYSIOLOGY,268.
MLA	Liu, Yu Ping,et al."Establishment of reference (housekeeping) genes via quantitative real-time PCR for investigation of the genomic basis of abiotic stress resistance in Psammochloa villosa (Poaceae)".JOURNAL OF PLANT PHYSIOLOGY 268(2022).