气候变化领域集成服务门户(CCPortal): Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B

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DOI	10.21037/atm-21-934
	Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B
	Shan, Zhongshu; Zhao, Yanyan; Qiu, Zhixue; Angxiu, Suonan; Gu, Yong; Luo, Junning; Bi, Hongtao; Luo, Wei; Xiong, Rui; Ma, Siqing; He, Zhao; Chen, Liang
通讯作者	Chen, L (通讯作者)，Soochow Univ, Affiliated Hosp 1, Dept Orthoped Surg, 188 Shizi St, Suzhou 215006, Peoples R China.
发表日期	2021
ISSN	2305-5839
EISSN	2305-5847
卷号	9 期号:6
英文摘要	Background: Ultraviolet B (UVB) has been reported to prevent bone loss by promoting the synthesis of vitamin D. However, UVB can also enhance osteoclastic differentiation, inhibit osteogenic differentiation, and cause oxidative damage. The present study aimed to analyze the osteoprotective effects of UVB and conjugated linoleic acid (CLA) in rats with ovariectomy-induced osteoporosis, and to determine the interactions between UVB and CLA and their effects on bone mesenchymal stem cells (BMSCs) and bone marrow mononuclear cells (BMMCs). Methods: In vitro, the distance of UVB irradiation and the dose of CLA were selected by immunofluorescence assays and Cytotoxicity assay. BMSCs and BMMCs were detected by immunohistochemical and immunofluorescence assays. In vivo, three-month-old female Sprague-Dawley rats that had undergone ovariectomy were treated with UVB and CLA. After 8 weeks of therapy, the femurs of the rats were examined by micro-computed tomography (CT) and immunohistochemical detection to assess the therapeutic efficacy. Results: The least inhibitive UVB distance and dosage of CLA were selected for the in vivo experiments. CLA effectively weakened the osteogenic inhibitory effect of UVB (72 cm), significantly improved the activity of alkaline phosphatase (ALP), promoted the formation of mineralized nodules, and alleviated the oxidative damage induced by UVB. CLA also effectively weakened the osteoclast-promoting effect of UVB (72 cm), inhibited osteoclast formation, and inhibited the inflammatory damage to BMMCs caused by UVB (72 cm) irradiation. Micro-CT results showed that UVB irradiation could promote bone formation in ovariectomized Sprague-Dawley rats, while CLA could significantly promote bone regeneration. Immunofluorescence assays results showed that CLA alleviated UVB-induced oxidative damage to osteoblasts. The ROS detection results demonstrated that CLA effectively alleviated UVB-induced oxidative damage to BMSCs. Furthermore, Immunohistochemical assays showed that UVB and CLA treatment increased bone density, inhibited osteolytic osteolysis, and enhanced osteogenic activity. Conclusions: CLA can effectively weaken osteoclast promotion, osteogenic inhibition, and oxidative damage caused by UVB. Combination treatment of UVB and CLA exerts an osteoprotective effect on ovariectomized osteoporotic rats and stimulates osteogenesis. The molecular mechanism of this interaction requires further investigation.
英文关键词	Ultraviolet light B (UVB); conjugated linoleic acid (CLA); osteogenic inhibition; osteoclast promotion; ovariectomized model rats
语种	英语
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS类目	Oncology ; Medicine, Research & Experimental
WOS记录号	WOS:000635089700016
来源期刊	ANNALS OF TRANSLATIONAL MEDICINE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254989
作者单位	[Shan, Zhongshu; Gu, Yong; Chen, Liang] Soochow Univ, Affiliated Hosp 1, Dept Orthoped Surg, 188 Shizi St, Suzhou 215006, Peoples R China; [Shan, Zhongshu; Zhao, Yanyan; Qiu, Zhixue; Angxiu, Suonan; He, Zhao] Peoples Hosp Qinghai Prov, Dept Orthoped Surg, Xining, Peoples R China; [Luo, Junning] Peoples Hosp Qinghai Prov, Dept Pathol, Xining, Peoples R China; [Bi, Hongtao] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Pharmacol & Safe, Xining, Peoples R China; [Luo, Wei] Peoples Hosp Qinghai Prov, Dept Endocrinol, Xining, Peoples R China; [Xiong, Rui] Peoples Hosp Qinghai Prov, Nutr Dept, Xining, Peoples R China; [Ma, Siqing] Peoples Hosp Qinghai Prov, Dept Crit Care Med, Xining, Peoples R China
推荐引用方式 GB/T 7714	Shan, Zhongshu,Zhao, Yanyan,Qiu, Zhixue,et al. Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B[J]. 中国科学院西北生态环境资源研究院,2021,9(6).
APA	Shan, Zhongshu.,Zhao, Yanyan.,Qiu, Zhixue.,Angxiu, Suonan.,Gu, Yong.,...&Chen, Liang.(2021).Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B.ANNALS OF TRANSLATIONAL MEDICINE,9(6).
MLA	Shan, Zhongshu,et al."Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B".ANNALS OF TRANSLATIONAL MEDICINE 9.6(2021).