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DOI	10.21037/atm-21-4215
	Profiles of messenger RNAs and MicroRNAs in hypoxia-induced hepatic stellate cells
	Zhang, Liting; Gao, Jing; Zhou, Dan; Wang, Xiaojun; Li, Junfeng; Wang, Juan; Chen, Hong; Xie, Xiaodong; Chen, Tuo
通讯作者	Chen, T (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoevironm & Resources, State Key Lab Cryospher Sci, Lanzhou 730000, Peoples R China. ; Chen, T (通讯作者)，Univ Chinese Acad Sci, Beijing 100049, Peoples R China. ; Xie, XD (通讯作者)，Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drug Gansu Prov, Lanzhou 730000, Peoples R China.
发表日期	2021
ISSN	2305-5839
EISSN	2305-5847
卷号	9 期号:18
英文摘要	Background: MicroRNA (miRNA) plays an important role in hepatic stellate cell (HSCs) activation and liver fibrosis. The purpose of this study is to explore the effect of hypoxia on the differential expression of mRNAs and miRNAs in rat HSCs. Methods: HSC-T6 cells were treated with cobalt chloride (CoCl2), and the activity of HSC-T6 cells was measured by the CCK-8 assay. The mRNA expression levels of hypoxia inducible factor-1 alpha (HIF-1 alpha), collagen type I, transforming growth factor-131 (TGF-131), and Smad7 were measured by RT-qPCR. The protein expression levels of HIF-1 alpha, Bax, Bcl-2, and caspase-3 were assayed by western blot. We used basal medium and 400 mu mol/L CoCl2 medium to treat HSC-T6 cells for 48 h. Cells were harvested after 48 h to extract RNA. Transcriptome sequencing was performed to investigate differentially expressed miRNAs and mRNAs (fold change >2; P<0.05). Bioinformatics analysis was performed to predict the functions of differentially expressed miRNAs and mRNAs. Further, we used RT-qPCR to detect the expression of mRNAs and miRNAs to confirm the accuracy of sequencing. Results: With the increase of CoCl2 concentration, the activity of HSC-T6 cells decreased (P<0.05). The mRNA expression levels of HIF-1 alpha, collagen I, TGF-131, and Smad7, and the protein expressions levels of HIF-1 alpha, Bax, caspase-3, and the Bcl-2/Bax ratio were increased compared with the control group (P<0.05), while the expression of Bcl-2 decreased. A total of 54 miRNAs (20 upregulated and 34 downregulated) and 1,423 mRNAs (685 upregulated and 738 downregulated) were differentially expressed in the 400 mu mol/L CoCl2 medium group compared to the control basal medium group. Further bioinformatics analysis demonstrated that the differentially expressed mRNAs and miRNAs were mainly enriched in the synthesis of extracellular matrix. In addition, we used RT-qPCR to detect the expression of mRNAs and miRNAs to confirm the accuracy of sequencing. Conclusions: Our results presented the profiles of mRNAs and miRNAs in hypoxia-induced HSC-T6 cells in rats, the signaling pathways, and co-expression networks. These findings may suggest novel insights for the early diagnosis and treatment of HSC activation and liver fibrosis.
关键词	INDUCIBLE FACTORS ACTIVATION PATHWAY EXPRESSION
英文关键词	Hypoxia; rat hepatic stellate cells (HSCs); genome sequencing; hypoxia inducible factor-1 alpha (HIF-1 alpha); miRNA
语种	英语
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS类目	Oncology ; Medicine, Research & Experimental
WOS记录号	WOS:000704380100017
来源期刊	ANNALS OF TRANSLATIONAL MEDICINE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254972
作者单位	[Zhang, Liting; Chen, Tuo] Chinese Acad Sci, Northwest Inst Ecoevironm & Resources, State Key Lab Cryospher Sci, Lanzhou 730000, Peoples R China; [Zhang, Liting; Chen, Tuo] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Liting; Li, Junfeng; Chen, Hong] Lanzhou Univ, Hosp 1, Dept Infect Dis, Lanzhou, Peoples R China; [Gao, Jing] Karolinska Inst, Dept Med, Resp Med Unit, Stockholm, Sweden; [Zhou, Dan] Lanzhou Univ, Hosp 1, Dept Liver Dis, Lanzhou, Peoples R China; [Wang, Xiaojun] Gansu Prov Hosp, Dept Resp Med, Lanzhou, Peoples R China; [Wang, Xiaojun] Lanzhou Univ, Sch Clin Med 1, Lanzhou, Peoples R China; [Wang, Juan; Chen, Hong] Xian Int Med Ctr, Dept Gastroenterol, Xian, Peoples R China; [Xie, Xiaodong] Lanzhou Univ, Inst Med Genet, Sch Basic Med Sci, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Liting,Gao, Jing,Zhou, Dan,et al. Profiles of messenger RNAs and MicroRNAs in hypoxia-induced hepatic stellate cells[J]. 中国科学院西北生态环境资源研究院,2021,9(18).
APA	Zhang, Liting.,Gao, Jing.,Zhou, Dan.,Wang, Xiaojun.,Li, Junfeng.,...&Chen, Tuo.(2021).Profiles of messenger RNAs and MicroRNAs in hypoxia-induced hepatic stellate cells.ANNALS OF TRANSLATIONAL MEDICINE,9(18).
MLA	Zhang, Liting,et al."Profiles of messenger RNAs and MicroRNAs in hypoxia-induced hepatic stellate cells".ANNALS OF TRANSLATIONAL MEDICINE 9.18(2021).