气候变化领域集成服务门户(CCPortal): The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

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DOI	10.3389/fvets.2021.754195
	The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep
	Cao, Yurong; Zhu, Baozhen; Li, Fei; Zhang, Duihong; Guo, Tongqing; Li, Fadi; Yang, Guo
通讯作者	Li, F (通讯作者)，Lanzhou Univ, Key Lab Grassland Livestock Ind Innovat,Minist Ed, State Key Lab Grassland Agroecosyst,Coll Pastoral, Minist Agr & Rural Affairs,Engn Res Ctr Grassland, Lanzhou, Peoples R China.
发表日期	2021
EISSN	2297-1769
卷号	8
英文摘要	This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 +/- 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P < 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.
关键词	CANNULATION FERMENTATION PARAMETERS SAMPLES
英文关键词	rumen fistula surgery; microbial diversity; sheep; rumen microbial function; BugBase function prediction
语种	英语
WOS研究方向	Veterinary Sciences
WOS类目	Veterinary Sciences
WOS记录号	WOS:000730148000001
来源期刊	FRONTIERS IN VETERINARY SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254970
作者单位	[Cao, Yurong; Zhu, Baozhen; Li, Fei; Zhang, Duihong; Guo, Tongqing; Li, Fadi] Lanzhou Univ, Key Lab Grassland Livestock Ind Innovat,Minist Ed, State Key Lab Grassland Agroecosyst,Coll Pastoral, Minist Agr & Rural Affairs,Engn Res Ctr Grassland, Lanzhou, Peoples R China; [Yang, Guo] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Cao, Yurong,Zhu, Baozhen,Li, Fei,et al. The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep[J]. 中国科学院西北生态环境资源研究院,2021,8.
APA	Cao, Yurong.,Zhu, Baozhen.,Li, Fei.,Zhang, Duihong.,Guo, Tongqing.,...&Yang, Guo.(2021).The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep.FRONTIERS IN VETERINARY SCIENCE,8.
MLA	Cao, Yurong,et al."The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep".FRONTIERS IN VETERINARY SCIENCE 8(2021).