气候变化领域集成服务门户(CCPortal): 12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching

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DOI	10.1016/j.talanta.2020.121788
	12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching
	Chen, Shi-En; Hu, Jingwen; Yan, Ping; Sun, Jing; Jia, Wenhui; Zhu, Shuyun; Zhao, Xian-En; Liu, Huwei
通讯作者	Zhao, XE (通讯作者)，Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Shandong, Peoples R China. ; Zhao, XE (通讯作者)，Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Nat Med Anal, Qufu 273165, Shandong, Peoples R China.
发表日期	2021
ISSN	0039-9140
EISSN	1873-3573
卷号	224
英文摘要	Urinary sarcosine was considered to be a potential biomarker for prostate cancer (Pca). In this work, an integrated strategy of multiplex tags chemical isotope labeling (MTCIL) combined with magnetic dispersive solid phase extraction (MDSPE), was proposed for specific extraction and high-throughput determination of sarcosine by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). In the past three months, we have developed 8-plex MTCIL reagents with excellent qualitative and quantitative performance. In this work, the multiplexing capacity of MTCIL reagents (MTCIL360/361/362/363/364/365/366/375/376/378/ 379/381) was increased 1.5-fold from 8-plex to 12-plex. MTCIL359 was prepared and used to label sarcosine standard as internal standard (IS). The structural analogue derivative (MTCIL373-sarcosine) of all targeted MTCIL-sarcosine derivatives was synthesized and used as a novel dummy template to prepare dummy magnetic molecularly imprinted polymers (DMMIPs). The integration of MTCIL and DMMIPs procedures were extremely favorable to excellent chromatographic separation and efficient mass spectrometric detection. The labeling efficiency, chromatographic retention and mass spectrometry responses of MTCIL reagents were consistent for sarcosine. In a single UHPLC-MS/MS run (2.0 min), this method can simultaneously quantify sarcosine in 12-plex urine samples and achieve unbiased concentrations comparison between different urine samples. Analytical parameters including linearity (R2 0.989-0.997), detection limits (0.02 nM), precision (2.6-11.5%), accuracy (96.1-107.4%), matrix effect, labeling and extraction efficiency were carefully validated. The proposed method was successfully applied for urinary sarcosine determination of healthy male individuals and Pca patients. It was found that the sarcosine concentrations in these two groups were statistically extremely significantly different (P < 0.001). The developed method was a powerful analytical tool to substantially promote the analysis throughput and large-scale experiments about the potential biomarker research.
关键词	SOLID-PHASE EXTRACTION GREEN SYNTHESIS STRATEGY PROSTATE-CANCER LIQUID-CHROMATOGRAPHY MAGNETIC COMPOSITES DERIVATIZATION BIOMARKERS HYDROXYPROLINE SEPARATION GAS
英文关键词	Sarcosine; Chemical isotope labeling; High throughput; Magnetic dispersive solid phase extraction; Liquid chromatography-tandem mass spectrometry; Prostate cancer
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000600787800021
来源期刊	TALANTA
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254957
作者单位	[Chen, Shi-En; Hu, Jingwen; Yan, Ping; Jia, Wenhui; Zhu, Shuyun; Zhao, Xian-En] Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Shandong, Peoples R China; [Chen, Shi-En; Hu, Jingwen; Yan, Ping; Jia, Wenhui; Zhu, Shuyun; Zhao, Xian-En] Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Nat Med Anal, Qufu 273165, Shandong, Peoples R China; [Sun, Jing] Chinese Acad Sci, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China; [Liu, Huwei] Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China; [Yan, Ping] Qufu Normal Univ, Univ Hosp, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Shi-En,Hu, Jingwen,Yan, Ping,et al. 12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching[J]. 中国科学院西北生态环境资源研究院,2021,224.
APA	Chen, Shi-En.,Hu, Jingwen.,Yan, Ping.,Sun, Jing.,Jia, Wenhui.,...&Liu, Huwei.(2021).12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching.TALANTA,224.
MLA	Chen, Shi-En,et al."12-Plex UHPLC-MS/MS analysis of sarcosine in human urine using integrated principle of multiplex tags chemical isotope labeling and selective imprint enriching".TALANTA 224(2021).