气候变化领域集成服务门户(CCPortal): Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK

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DOI	10.1080/21505594.2021.1958042
	Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK
	Han, Xuelin; Su, Xueting; Li, Zhiqian; Liu, Yanxi; Wang, Shuo; Zhu, Miao; Zhang, Changjian; Yang, Fan; Zhao, Jingya; Li, Xianping; Chen, Fangyan; Han, Li
通讯作者	Chen, FY ; Han, L (通讯作者)，Chinese PLA Ctr Dis Control & Prevent, Dept Disinfect & Infect Control, Beijing, Peoples R China.
发表日期	2021
ISSN	2150-5594
EISSN	2150-5608
起始页码	1980
结束页码	1996
卷号	12 期号:1
英文摘要	Complement receptor 3 (CD11b/CD18) is an important receptor that mediates adhesion, phagocytosis and chemotaxis in various immunocytes. The conidia of the medically-important pathogenic fungus, Aspergillus fumigatus can be internalized into alveolar epithelial cells to disseminate its infection in immunocompromised host; however, the role of CR3 in this process is poorly understood. In the present study, we investigated the potential role of CR3 on A. fumigatus internalization into type II alveolar epithelial cells and its effect on host intracellular PA content induced by A. fumigatus. We found that CR3 is expressed in alveolar epithelial cells and that human serum and bronchoalveolar lavage fluid (BALF) could improve A. fumigatus conidial internalization into A549 type II alveolar epithelial cell line and mouse primary alveolar epithelial cells, which were significantly inhibited by the complement C3 quencher and CD11b-blocking antibody. Serum-opsonization of swollen conidia, but not resting conidia led to the increase of cellular phosphatidic acid (PA) in A549 cells during infection. Moreover, both conidial internalization and induced PA production were interfered by CD11b-blocking antibody and dependent on FAK activity, but not Syk in alveolar epithelial cells. Overall, our results revealed that CR3 is a critical modulator of Aspergillus fumigatus internalization into alveolar epithelial cells.
关键词	LECTIN-LIKE DOMAIN PHOSPHOLIPASE-D MYCOBACTERIUM-TUBERCULOSIS TYROSINE KINASE BINDING PHAGOCYTOSIS NEUTROPHILS CD11B/CD18 INTEGRIN OPSONIZATION
英文关键词	Aspergillus fumigatus; Complement receptor 3; alveolar epithelial cells; opsonization; phosphatidic acid; FAK
语种	英语
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:000680215600001
来源期刊	VIRULENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254914
作者单位	[Han, Xuelin; Su, Xueting; Li, Zhiqian; Liu, Yanxi; Wang, Shuo; Zhu, Miao; Zhang, Changjian; Yang, Fan; Zhao, Jingya; Chen, Fangyan; Han, Li] Chinese PLA Ctr Dis Control & Prevent, Dept Disinfect & Infect Control, Beijing, Peoples R China; [Li, Zhiqian] 907th Hosp, Dept Lab Med & Blood Transfus, Nanping, Fujian, Peoples R China; [Wang, Shuo] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China; [Zhang, Changjian] Peoples Liberat Army Gen Hosp, Cent Lab, Med Ctr 6, Beijing, Peoples R China; [Li, Xianping] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Han, Xuelin,Su, Xueting,Li, Zhiqian,et al. Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK[J]. 中国科学院西北生态环境资源研究院,2021,12(1).
APA	Han, Xuelin.,Su, Xueting.,Li, Zhiqian.,Liu, Yanxi.,Wang, Shuo.,...&Han, Li.(2021).Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK.VIRULENCE,12(1).
MLA	Han, Xuelin,et al."Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK".VIRULENCE 12.1(2021).