气候变化领域集成服务门户(CCPortal): Chemerin reverses the malignant phenotype and induces differentiation of human hepatoma SMMC7721 cells

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DOI	10.1007/s12272-021-01311-z
	Chemerin reverses the malignant phenotype and induces differentiation of human hepatoma SMMC7721 cells
	Li, Ming; Sun, Pengcheng; Dong, Kaikai; Xin, Ye; TaiLulu, Aslee; Li, Qinyu; Sun, Jing; Peng, Min; Shi, Ping
通讯作者	Shi, P (通讯作者)，East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China. ; Li, QY (通讯作者)，Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Gen Surg, Sch Med, 197 Ruijin Er Rd, Shanghai 200025, Peoples R China. ; Shi, P (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xiguan Ave 59, Xining 810001, Peoples R China.
发表日期	2021
ISSN	0253-6269
EISSN	1976-3786
起始页码	194
结束页码	204
卷号	44 期号:2
英文摘要	Chemerin exhibits an inhibitory effect on hepatocellular carcinoma; however, the underlying mechanism is unclear. Here, low chemerin expression was confirmed in samples of liver cancer patients and hepatoma cells. Chemerin altered hepatoma cell morphology but had no effect on normal hepatocytes. Chemerin inhibited proliferation of several human hepatoma cell lines. Real-time PCR detection of hepatocellular carcinoma markers showed that mRNA levels of albumin and A-type gamma-glutamyl transferase increased whereas those of alpha-fetoprotein, alkaline phosphatase, B-type gamma-glutamyl transferase, insulin-like growth factor II, and human telomerase reverse transcriptase decreased in chemerin-treated SMMC7721 cells. Western blotting revealed that chemerin up-regulated albumin and vimentin expressions, and downregulated alpha-fetoprotein expression. Phosphorylated STAT3 was significantly up-regulated, whereas phosphorylated ERK and AKT were significantly downregulated by chemerin. Chemerin decreased phosphorylated ERK and AKT expression and the cell proliferation induced by PI3K activator 740 Y-P but could not significantly alter phosphorylated STAT3 expression and the cell growth induced by STAT3 inhibitor NSC74859. In conclusion, chemerin reversed the malignant phenotype and induced SMMC7721 cell differentiation by inhibiting MAPK/ERK and PI3K/AKT signaling; growth inhibition by chemerin is not directly related to the JAK/STAT signaling pathway. Our study provides novel evidence that chemerin could be utilized for liver cancer treatment.
关键词	TRANSPEPTIDASE MESSENGER-RNA HEPATOCELLULAR-CARCINOMA SIGNAL TRANSDUCER ACTIVATION GROWTH CANCER STAT3 SUPPRESSES EXPRESSION INDICATOR
英文关键词	Chemerin; Human hepatic carcinoma SMMC7721 cells; Cell proliferation; Malignant phenotype; Cell differentiation
语种	英语
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号	WOS:000612269800001
来源期刊	ARCHIVES OF PHARMACAL RESEARCH
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254866
作者单位	[Li, Ming; Sun, Pengcheng; Dong, Kaikai; Xin, Ye; TaiLulu, Aslee; Shi, Ping] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China; [Li, Qinyu] Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Gen Surg, Sch Med, 197 Ruijin Er Rd, Shanghai 200025, Peoples R China; [Sun, Jing; Peng, Min; Shi, Ping] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xiguan Ave 59, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Li, Ming,Sun, Pengcheng,Dong, Kaikai,et al. Chemerin reverses the malignant phenotype and induces differentiation of human hepatoma SMMC7721 cells[J]. 中国科学院西北生态环境资源研究院,2021,44(2).
APA	Li, Ming.,Sun, Pengcheng.,Dong, Kaikai.,Xin, Ye.,TaiLulu, Aslee.,...&Shi, Ping.(2021).Chemerin reverses the malignant phenotype and induces differentiation of human hepatoma SMMC7721 cells.ARCHIVES OF PHARMACAL RESEARCH,44(2).
MLA	Li, Ming,et al."Chemerin reverses the malignant phenotype and induces differentiation of human hepatoma SMMC7721 cells".ARCHIVES OF PHARMACAL RESEARCH 44.2(2021).