气候变化领域集成服务门户(CCPortal): Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring

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DOI	10.1016/j.foodchem.2022.132390
	Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring
	Liu, Sijie; Shu, Rui; Nie, Chen; Li, Yuechun; Luo, Xing; Ji, Yanwei; Yin, Xuechi; Sun, Jing; Zhang, Daohong; Wang, Jianlong
通讯作者	Wang, JL (通讯作者)，Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China.
发表日期	2022
ISSN	0308-8146
EISSN	1873-7072
卷号	382
英文摘要	Although lateral flow immunoassay (LFIA) as a point-of-care (POC) diagnostic tool has been widely used because of numerous merits, it remains challenging to realize higher sensitivity, easier labeling, and fewer antibodies consumption. Herein, a bioresource-derived tannic acid (TA)-supported immuno-network based LFIA for clenbuterol (CLE) monitoring was proposed by employing poly TA nanospheres (PTAN) as the new tracer. Attributing to the effective protein-enrichment ability of TA, plenty of goat anti-mouse immunoglobulins (GAMI) were first absorbed on the surface of PTAN and then monoclonal antibodies (Ab(CLE)) were added to form immuno-network. Compared with directly coupling antibodies, the utilization efficiency of Ab(CLE) was significantly improved. Thus, the detection sensitivity (take the cut-off value as an example) was enhanced 5-fold and 10-fold at least compared with PTAN-Ab(CLE) and colloidal gold-based LFIA, respectively. Additionally, the proposed method was successfully verified in beef and pork liver and provided an effective strategy for food safety monitoring.
关键词	IMMUNOCHROMATOGRAPHIC ASSAY RAPID DETECTION NANOPARTICLES POINT
英文关键词	Tannic acid; Lateral flow immunoassay; Bioresource-derived; Immuno-network; Clenbuterol
语种	英语
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS类目	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000777259200003
来源期刊	FOOD CHEMISTRY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254828
作者单位	[Liu, Sijie; Shu, Rui; Nie, Chen; Li, Yuechun; Luo, Xing; Ji, Yanwei; Yin, Xuechi; Zhang, Daohong; Wang, Jianlong] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China; [Sun, Jing] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Qinghai Tibet Plateau Biol R, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Liu, Sijie,Shu, Rui,Nie, Chen,et al. Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring[J]. 中国科学院西北生态环境资源研究院,2022,382.
APA	Liu, Sijie.,Shu, Rui.,Nie, Chen.,Li, Yuechun.,Luo, Xing.,...&Wang, Jianlong.(2022).Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring.FOOD CHEMISTRY,382.
MLA	Liu, Sijie,et al."Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring".FOOD CHEMISTRY 382(2022).