气候变化领域集成服务门户(CCPortal): Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration

CCPortal

DOI	10.24272/j.issn.2095-8137.2021.195
	Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration
	Sun, Kuan-Xiang; Jiang, Xiao-Yan; Li, Xiao; Su, Yu-Jing; Wang, Ju-Lin; Zhang, Lin; Yang, Ye-Ming; Zhu, Xian-Jun
通讯作者	Zhu, XJ (通讯作者)，Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Hlth Management Ctr, Sch Med, Chengdu 610072, Sichuan, Peoples R China. ; Zhu, XJ (通讯作者)，Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Ctr Med Genet, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China. ; Zhu, XJ (通讯作者)，Sichuan Acad Med Sci & Sichuan Prov Peoples Hosp, Res Unit Blindness Prevent, Chinese Acad Med Sci, Chengdu 610072, Sichuan, Peoples R China. ; Zhu, XJ (通讯作者)，First Peoples Hosp Shangqiu, Dept Ophthalmol, Shangqiu 476000, Henan, Peoples R China. ; Zhu, XJ (通讯作者)，Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China. ; Zhu, XJ (通讯作者)，Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China.
发表日期	2021
ISSN	2095-8137
起始页码	650
结束页码	+
卷号	42 期号:5
英文摘要	Phosphatidylserine (PS) is distributed asymmetrically in the plasma membrane of eukaryotic cells. Phosphatidylserine flippase (P4-ATPase) transports PS from the outer leaflet of the lipid bilayer to the inner leaflet of the membrane to maintain PS asymmetry. The beta subunit TMEM30A is indispensable for transport and proper function of P4-ATPase. Previous studies have shown that the ATP11A and TMEM30A complex is the molecular switch for myotube formation. However, the role of Tmem30a in skeletal muscle regeneration remains elusive. In the current study, Tmem30a was highly expressed in the tibialis anterior (TA) muscles of dystrophin-null (mdx) mice and BaCl2-induced muscle injury model mice. We generated a satellite cell (SC)-specific Tmem30a conditional knockout (cKO) mouse model to investigate the role of Tmem30a in skeletal muscle regeneration. The regenerative ability of cKO mice was evaluated by analyzing the number and diameter of regenerated SCs after the TA muscles were injured by BaCl2-injection. Compared to the control mice, the cKO mice showed decreased Pax7(+) and MYH3(+) SCs, indicating diminished SC proliferation, and decreased expression of muscular regulatory factors (MYOD and MYOG), suggesting impaired myoblast proliferation in skeletal muscle regeneration. Taken together, these results demonstrate the essential role of Tmem30a in skeletal muscle regeneration.
关键词	PHOSPHOLIPID FLIPPASE MUSCULAR-DYSTROPHY CDC50 PROTEINS STEM-CELLS FIBROBLASTS EXPRESSION TRANSPORT PROLIFERATION LOCALIZATION MAINTENANCE
英文关键词	Tmem30a; Skeletal muscle regeneration; Knockout mouse model Atp11a; Satellite cell
语种	英语
WOS研究方向	Zoology
WOS类目	Zoology
WOS记录号	WOS:000702272000011
来源期刊	ZOOLOGICAL RESEARCH
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254798
作者单位	[Sun, Kuan-Xiang; Jiang, Xiao-Yan; Li, Xiao; Zhu, Xian-Jun] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Hlth Management Ctr, Sch Med, Chengdu 610072, Sichuan, Peoples R China; [Sun, Kuan-Xiang; Jiang, Xiao-Yan; Li, Xiao; Su, Yu-Jing; Wang, Ju-Lin; Zhang, Lin; Yang, Ye-Ming; Zhu, Xian-Jun] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Ctr Med Genet, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu 610072, Sichuan, Peoples R China; [Sun, Kuan-Xiang; Zhu, Xian-Jun] Sichuan Acad Med Sci & Sichuan Prov Peoples Hosp, Res Unit Blindness Prevent, Chinese Acad Med Sci, Chengdu 610072, Sichuan, Peoples R China; [Zhu, Xian-Jun] First Peoples Hosp Shangqiu, Dept Ophthalmol, Shangqiu 476000, Henan, Peoples R China; [Zhu, Xian-Jun] Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China; [Zhu, Xian-Jun] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Sun, Kuan-Xiang,Jiang, Xiao-Yan,Li, Xiao,et al. Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration[J]. 中国科学院西北生态环境资源研究院,2021,42(5).
APA	Sun, Kuan-Xiang.,Jiang, Xiao-Yan.,Li, Xiao.,Su, Yu-Jing.,Wang, Ju-Lin.,...&Zhu, Xian-Jun.(2021).Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration.ZOOLOGICAL RESEARCH,42(5).
MLA	Sun, Kuan-Xiang,et al."Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration".ZOOLOGICAL RESEARCH 42.5(2021).