气候变化领域集成服务门户(CCPortal): Copy number variation of the CCDC39 gene is associated with growth traits in Chinese cattle

CCPortal

DOI	10.1002/vms3.712
	Copy number variation of the CCDC39 gene is associated with growth traits in Chinese cattle
	Hu, Linyong; Yu, Junjian; Huang, Rong; Yang, Peng; Zhang, Zijing; Chai, Yanan; Shi, Qiaoting; Chen, Fuying; Liu, Xian; Li, Zhiming; Ru, Baorui; Wang, Eryao; Lei, Chuzhao; Peng, Wei; Huang, Yongzhen
通讯作者	Huang, YZ (通讯作者)，Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. ; Peng, W (通讯作者)，Qinghai Univ, Qinghai Acad Anim Sci & Vet Med, Xining 810016, Qinghai, Peoples R China.
发表日期	2022
EISSN	2053-1095
起始页码	917
结束页码	924
卷号	8 期号:2
英文摘要	Background: Copy number variation (CNV) has become an essential part of genetic structural variation. Coiled-coil domain containing 39 (CCDC39) is a gene that related to the growth and development of organs and tissues. It is identified that it has a CNV region by animal genome resequencing. Objective: In this study, we detected the phenotypic traits and different distributions of CCDC39 gene copy numbers in five Chinese cattle breeds (Qinchuan (QC) cattle, Yunling (YL) cattle, Xianan (XN) cattle, Pinan (PN) cattle and Jiaxian (JX) cattle). Methods: Five hundred and six cattle were randomly selected for CNV distribution detection. Blood samples were taken and genomic DNA was extracted. Different tissues were obtained from adult (n = 3) XN cattle, including heart, liver, kidney, skeletal muscle and lung. The genome qPCR experiment was performed with SYBR Green in triplicate. CDNA qPCR was used to detect the expression level of CCDC39 in different tissues and varieties. Using SPSS v20.0 software, the relationship between CCDC39 CNV and the growth traits of PN, XN, QC, NY and YL cattle breeds was analyzed by one-way analysis of variance (ANOVA). Results: The results showed that the expression of CCDC39 in lung was higher than that in other tissues. The expression in liver and kidney was similar, but the expression in heart and muscle was less. It can be seen that the duplication type of QC cattle CCDC39 CNV is higher than the deletion or normal in the height at hip cross. The normal type of PN cattle in body length and hip width was better than duplication and deletion (p < 0.05). In XN cattle, the deletion type of CNV had superior growth characteristics in heart girth and cannon bone circumference compared with the duplication type and the normal type (p <0.05). Conclusion: The study revealed a significant association between CNV of CCDC39 gene and growth traits in different Chinese cattle breeds.
关键词	POLYMORPHISM GENOME PCR
英文关键词	association; CCDC39 gene; Chinese beef cattle; copy number variations (CNV); growth trait; mRNA expression
语种	英语
WOS研究方向	Veterinary Sciences
WOS类目	Veterinary Sciences
WOS记录号	WOS:000762774500001
来源期刊	VETERINARY MEDICINE AND SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254756
作者单位	[Hu, Linyong; Lei, Chuzhao] Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China; [Yu, Junjian; Yang, Peng; Huang, Yongzhen] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China; [Huang, Rong; Peng, Wei] Qinghai Univ, Qinghai Acad Anim Sci & Vet Med, Xining 810016, Qinghai, Peoples R China; [Zhang, Zijing; Chai, Yanan; Shi, Qiaoting; Chen, Fuying; Wang, Eryao] Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou, Henan, Peoples R China; [Liu, Xian; Li, Zhiming; Ru, Baorui] Henan Prov Anim Husb Gen Stn, Zhengzhou, Henan, Peoples R China
推荐引用方式 GB/T 7714	Hu, Linyong,Yu, Junjian,Huang, Rong,et al. Copy number variation of the CCDC39 gene is associated with growth traits in Chinese cattle[J]. 中国科学院西北生态环境资源研究院,2022,8(2).
APA	Hu, Linyong.,Yu, Junjian.,Huang, Rong.,Yang, Peng.,Zhang, Zijing.,...&Huang, Yongzhen.(2022).Copy number variation of the CCDC39 gene is associated with growth traits in Chinese cattle.VETERINARY MEDICINE AND SCIENCE,8(2).
MLA	Hu, Linyong,et al."Copy number variation of the CCDC39 gene is associated with growth traits in Chinese cattle".VETERINARY MEDICINE AND SCIENCE 8.2(2022).