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DOI | 10.1002/cjoc.202100070 |
Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin | |
Wang, Jinxiu; Ma, Suze; Ding, Wei; Chen, Tuo; Zhang, Qi | |
通讯作者 | Chen, T (通讯作者),Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China. ; Zhang, Q (通讯作者),Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China. |
发表日期 | 2021 |
ISSN | 1001-604X |
EISSN | 1614-7065 |
起始页码 | 1923 |
结束页码 | 1926 |
卷号 | 39期号:7 |
英文摘要 | Main observation and conclusion The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety, and biosynthesis of this moiety involves an oxidoreductase AprQ. Unlike other known Q series proteins involved in aminoglycosides biosynthesis, AprQ does not work with an aminotransferase partner, and performs a four-electron oxidation that converts a CH2OH moiety to a carboxylate group. In this study, we report mechanistic investigation of AprQ. We showed AprQ contains a flavin mononucleotide (FMN) cofactor, which is different from other known Q series enzymes that contain a flavin adenine dinucleotide (FAD) cofactor. A series of biochemical assays showed that AprQ is not a monooxygenase but a flavoprotein oxidase. Although molecular O-2 is strictly required for reaction turnover, four-electron oxidation can be achieved in the absence of O-2 in single turnover condition. These findings establish the detailed catalytic mechanism of AprQ and expand the growing family of flavoprotein oxidases, an increasingly important class of biocatalysts. |
关键词 | ENZYME APRD4BRANCH POINTSPECIFICITYNEOMYCIN |
英文关键词 | Biosynthesis; Flavin; Flavoprotein; Oxidase; Enzyme mechanism |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000655023200001 |
来源期刊 | CHINESE JOURNAL OF CHEMISTRY
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来源机构 | 中国科学院西北生态环境资源研究院 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/254697 |
作者单位 | [Wang, Jinxiu; Chen, Tuo] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China; [Wang, Jinxiu; Ma, Suze; Zhang, Qi] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China; [Wang, Jinxiu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ding, Wei] Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Jinxiu,Ma, Suze,Ding, Wei,et al. Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin[J]. 中国科学院西北生态环境资源研究院,2021,39(7). |
APA | Wang, Jinxiu,Ma, Suze,Ding, Wei,Chen, Tuo,&Zhang, Qi.(2021).Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin.CHINESE JOURNAL OF CHEMISTRY,39(7). |
MLA | Wang, Jinxiu,et al."Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin".CHINESE JOURNAL OF CHEMISTRY 39.7(2021). |
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