CCPortal
DOI10.1002/cjoc.202100070
Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin
Wang, Jinxiu; Ma, Suze; Ding, Wei; Chen, Tuo; Zhang, Qi
通讯作者Chen, T (通讯作者),Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China. ; Zhang, Q (通讯作者),Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.
发表日期2021
ISSN1001-604X
EISSN1614-7065
起始页码1923
结束页码1926
卷号39期号:7
英文摘要Main observation and conclusion The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety, and biosynthesis of this moiety involves an oxidoreductase AprQ. Unlike other known Q series proteins involved in aminoglycosides biosynthesis, AprQ does not work with an aminotransferase partner, and performs a four-electron oxidation that converts a CH2OH moiety to a carboxylate group. In this study, we report mechanistic investigation of AprQ. We showed AprQ contains a flavin mononucleotide (FMN) cofactor, which is different from other known Q series enzymes that contain a flavin adenine dinucleotide (FAD) cofactor. A series of biochemical assays showed that AprQ is not a monooxygenase but a flavoprotein oxidase. Although molecular O-2 is strictly required for reaction turnover, four-electron oxidation can be achieved in the absence of O-2 in single turnover condition. These findings establish the detailed catalytic mechanism of AprQ and expand the growing family of flavoprotein oxidases, an increasingly important class of biocatalysts.
关键词ENZYME APRD4BRANCH POINTSPECIFICITYNEOMYCIN
英文关键词Biosynthesis; Flavin; Flavoprotein; Oxidase; Enzyme mechanism
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000655023200001
来源期刊CHINESE JOURNAL OF CHEMISTRY
来源机构中国科学院西北生态环境资源研究院
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/254697
作者单位[Wang, Jinxiu; Chen, Tuo] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China; [Wang, Jinxiu; Ma, Suze; Zhang, Qi] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China; [Wang, Jinxiu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ding, Wei] Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
推荐引用方式
GB/T 7714
Wang, Jinxiu,Ma, Suze,Ding, Wei,et al. Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin[J]. 中国科学院西北生态环境资源研究院,2021,39(7).
APA Wang, Jinxiu,Ma, Suze,Ding, Wei,Chen, Tuo,&Zhang, Qi.(2021).Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin.CHINESE JOURNAL OF CHEMISTRY,39(7).
MLA Wang, Jinxiu,et al."Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin".CHINESE JOURNAL OF CHEMISTRY 39.7(2021).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Jinxiu]的文章
[Ma, Suze]的文章
[Ding, Wei]的文章
百度学术
百度学术中相似的文章
[Wang, Jinxiu]的文章
[Ma, Suze]的文章
[Ding, Wei]的文章
必应学术
必应学术中相似的文章
[Wang, Jinxiu]的文章
[Ma, Suze]的文章
[Ding, Wei]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。