气候变化领域集成服务门户(CCPortal): Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin

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DOI	10.1002/cjoc.202100070
	Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin
	Wang, Jinxiu; Ma, Suze; Ding, Wei; Chen, Tuo; Zhang, Qi
通讯作者	Chen, T (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China. ; Zhang, Q (通讯作者)，Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.
发表日期	2021
ISSN	1001-604X
EISSN	1614-7065
起始页码	1923
结束页码	1926
卷号	39 期号:7
英文摘要	Main observation and conclusion The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety, and biosynthesis of this moiety involves an oxidoreductase AprQ. Unlike other known Q series proteins involved in aminoglycosides biosynthesis, AprQ does not work with an aminotransferase partner, and performs a four-electron oxidation that converts a CH2OH moiety to a carboxylate group. In this study, we report mechanistic investigation of AprQ. We showed AprQ contains a flavin mononucleotide (FMN) cofactor, which is different from other known Q series enzymes that contain a flavin adenine dinucleotide (FAD) cofactor. A series of biochemical assays showed that AprQ is not a monooxygenase but a flavoprotein oxidase. Although molecular O-2 is strictly required for reaction turnover, four-electron oxidation can be achieved in the absence of O-2 in single turnover condition. These findings establish the detailed catalytic mechanism of AprQ and expand the growing family of flavoprotein oxidases, an increasingly important class of biocatalysts.
关键词	ENZYME APRD4 BRANCH POINT SPECIFICITY NEOMYCIN
英文关键词	Biosynthesis; Flavin; Flavoprotein; Oxidase; Enzyme mechanism
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000655023200001
来源期刊	CHINESE JOURNAL OF CHEMISTRY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254697
作者单位	[Wang, Jinxiu; Chen, Tuo] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Cryospher Sci, Key Lab Extreme Environm Microbial Resources & En, Lanzhou 730000, Gansu, Peoples R China; [Wang, Jinxiu; Ma, Suze; Zhang, Qi] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China; [Wang, Jinxiu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ding, Wei] Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jinxiu,Ma, Suze,Ding, Wei,et al. Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin[J]. 中国科学院西北生态环境资源研究院,2021,39(7).
APA	Wang, Jinxiu,Ma, Suze,Ding, Wei,Chen, Tuo,&Zhang, Qi.(2021).Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin.CHINESE JOURNAL OF CHEMISTRY,39(7).
MLA	Wang, Jinxiu,et al."Mechanistic Study of Oxidoreductase AprQ Involved in Biosynthesis of Aminoglycoside Antibiotic Apramycin".CHINESE JOURNAL OF CHEMISTRY 39.7(2021).