气候变化领域集成服务门户(CCPortal): Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells

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DOI	10.3390/ijms222112009
	Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells
	Han, Yu; Yuan, Chen; Zhou, Xiaowei; Han, Yingjie; He, Yanhao; Ouyang, Jian; Zhou, Wenna; Wang, Zhenhua; Wang, Honglun; Li, Gang
通讯作者	Li, G (通讯作者)，Yantai Univ, Ctr Mitochondria & Hlth Aging, Coll Life Sci, Yantai 264005, Peoples R China.
发表日期	2021
EISSN	1422-0067
卷号	22 期号:21
英文摘要	Oleanolic acid (OA), asiatic acid (AA), and maslinic acid (MA) are ubiquitous isomeric triterpene phytochemicals with many pharmacological effects. To improve their application value, we used lipopolysaccharide (LPS) to induce RAW264.7 cells and studied the differences in the anti-inflammatory effects of the triterpenes according to their structural differences. MTT, Griess, and immunofluorescence assays, ELISA, flow cytometry, and Western blotting, were performed. The release of LPS-induced pro-inflammatory mediators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), and interleukin (IL-6), was significantly inhibited by OA, AA, and MA at the same concentration, and AA and MA promoted the production of anti-inflammatory factor IL-10. OA, AA, and MA inhibited LPS-induced NF-kappa B nuclear translocation in RAW264.7 cells. OA and AA inhibited the phosphorylation of ERK1/2, P38, and JNK1/2 in LPS-stimulated RAW264.7 cells. Moreover, OA increased LPS-induced Nrf2 expression and decreased Keap1 expression in RAW264.7 cells. OA, AA, and MA inhibited LPS-stimulated intracellular reactive oxygen species (ROS) production and alleviated mitochondrial membrane potential depletion. Overall, our data suggested that OA, AA, and MA exhibited significant anti-inflammatory effects in vitro. In particular, OA and AA take effects through the MAPKs, NF-kappa B, and Nrf2 signaling pathways.
关键词	NF-KAPPA-B CYCLOCARYA-PALIURUS POLYSACCHARIDE INFLAMMATORY RESPONSE ACTIVATION PATHWAY
英文关键词	inflammation; RAW264; 7 cell; oleanolic acid; asiatic acid; maslinic acid; NF-kappa B
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000719007400001
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254589
作者单位	[Han, Yu; Yuan, Chen; Zhou, Xiaowei; Han, Yingjie; He, Yanhao; Wang, Zhenhua; Li, Gang] Yantai Univ, Ctr Mitochondria & Hlth Aging, Coll Life Sci, Yantai 264005, Peoples R China; [Ouyang, Jian; Wang, Honglun] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China; [Zhou, Wenna] Huzhou Univ, Dept Life Sci & Hlth, QiuZhen Coll, Huzhou 313000, Peoples R China
推荐引用方式 GB/T 7714	Han, Yu,Yuan, Chen,Zhou, Xiaowei,et al. Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells[J]. 中国科学院西北生态环境资源研究院,2021,22(21).
APA	Han, Yu.,Yuan, Chen.,Zhou, Xiaowei.,Han, Yingjie.,He, Yanhao.,...&Li, Gang.(2021).Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(21).
MLA	Han, Yu,et al."Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.21(2021).