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DOI	10.1016/j.chroma.2021.462582
	The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example
	Wang, Qiqi; Chen, Tao; Cui, Yunbin; Li, Si; Jiang, Xinhao; Zhao, Guodong; Li, Yulin; Zou, Denglang
通讯作者	Li, YL (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China. ; Zou, DL (通讯作者)，Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Sch Life Sci, Key Lab Biodivers Format Mech & Comprehens Utiliz, Xining 810000, Peoples R China.
发表日期	2021
ISSN	0021-9673
EISSN	1873-3778
卷号	1657
英文摘要	Biosynthesis is a research hot-spot in recent years, however, the purification of its final products is a tough work. Liquid stationary phase and large-scale separation ability of PZRCCC could easily avoid the commonly disadvantages occurred in traditional column chromatography. These characteristics makes PZRCCC particularly applicable for final products separation in biosynthesis. In this study, the glycosylation products of ellagic acid by one-pot glycosylation were successfully purified by PZRCCC to show the applicability of PZRCCC for preparative separation of biosynthesis products. An optimized ethyl acetate/n-buthanol/water (3:3:5, v/v/v) system was applied in this study, where 5 mM trifluoroacetic acid (TFA) as the retainer and 30 mM triethylamine (TEA) as the eluter were added. As a result, four ellagic acid glycosylation products, including 51 mg of ellagic acid-4, 3'-O-beta-D-diglucoside (EG-1), 24 mg of ellagic acid-4, 4'-O-beta-D-diglucoside (EG-2), 11 mg of ellagic acid-4-O-beta-D-glucosyl (1 -> 2)-beta-D-glucoside (EG-3) and 64 mg of ellagic acid-4-O-beta-D-glucoside (EG-4) were simultaneously separated from 500 mg of glycosylation crude products, with the purity of 93.3%, 91.2%, 89.4% and 95.5%, respectively. Their structures were identified by spectroscopic analysis. (C) 2021 Elsevier B.V. All rights reserved.
关键词	ESCHERICHIA-COLI NATURAL-PRODUCTS EFFICIENT STRATEGY SYNTHETIC BIOLOGY GLYCOSYLTRANSFERASE BUFADIENOLIDE EXTRACTION CHEMISTRY
英文关键词	PH-zone-refining counter-current chromatography; Biosynthesis; Glycosylation; Ellagic acid
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000704584000007
来源期刊	JOURNAL OF CHROMATOGRAPHY A
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254576
作者单位	[Wang, Qiqi] Jinan Univ, Coll Pharm, Guangzhou 510632, Peoples R China; [Chen, Tao; Li, Yulin] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China; [Cui, Yunbin; Li, Si; Jiang, Xinhao; Zhao, Guodong; Zou, Denglang] Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Sch Life Sci, Key Lab Biodivers Format Mech & Comprehens Utiliz, Xining 810000, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qiqi,Chen, Tao,Cui, Yunbin,et al. The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example[J]. 中国科学院西北生态环境资源研究院,2021,1657.
APA	Wang, Qiqi.,Chen, Tao.,Cui, Yunbin.,Li, Si.,Jiang, Xinhao.,...&Zou, Denglang.(2021).The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example.JOURNAL OF CHROMATOGRAPHY A,1657.
MLA	Wang, Qiqi,et al."The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example".JOURNAL OF CHROMATOGRAPHY A 1657(2021).