气候变化领域集成服务门户(CCPortal): Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography

CCPortal

DOI	10.1039/d1ra05819c
	Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography
	Dang, Jun; Ma, Jianbin; Dawa, Yangzom; Liu, Chuang; Ji, Tengfei; Wang, Qilan
通讯作者	Wang, QL (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China. ; Ji, TF (通讯作者)，Chinese Acad Med Sci, Inst Mat Med, Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Medic, Beijing 100050, Peoples R China.
发表日期	2021
EISSN	2046-2069
起始页码	38739
结束页码	38749
卷号	11 期号:61
英文摘要	Traditional Tibetan medicines elaborately document the health benefits of Saxifraga sinomontana. However, there have been limited reports on its chemical make-up, presumably because of the complicated separation and purification process. In this work, a methanolic extract of Saxifraga sinomontana was utilized for targeted separation of 4 key 1,1-diphenyl-2-picrylhydrazyl inhibitors employing the medium-pressure liquid chromatography, reversed-phase liquid chromatography in combination with on-line reversed-phase liquid chromatography-1,1-diphenyl-2-picrylhydrazyl detection. Pre-treatment of the sample was carried out by employing medium-pressure liquid chromatography using MCI GEL (R) CHP20P as the stationary phase, furnishing 2.4 g of fraction Fr3 and 3.4 g of fraction Fr4 (the percentage retrieval was 32.7%). The 1,1-diphenyl-2-picrylhydrazyl inhibitors contained in fractions Fr3 and Fr4 were subjected to additional separation using a C18 (ReproSil-Pur C18 AQ) column and yielded 106.2 mg of Fr3-1, 246.9 mg of Fr3-2, 248.5 mg of Fr4-1 and 41.8 mg of Fr4-2. The degree of purity, structures and 1,1-diphenyl-2-picrylhydrazyl inhibition activity of the isolated DPPH inhibitors were determined, and four 1,1-diphenyl-2-picrylhydrazyl inhibitors including two new diarylnonanoids (3-methoxy-4-hydroxyphenol-(6 '-O-galloyl)-1-O-beta-d-glucopyrano side with IC50 of 39.6 mu M, 3,4,5-trimethoxyphenyl-(6 '-O-galloyl)-1-O-beta-d-glucopyranoside with IC50 of 46.9 mu M, saximonsin A with IC50 of 11.4 mu M, and saximonsin B with IC50 of 20.6 mu M) were isolated with a percentage purity above 95%. The methodology thus evolved has good efficacy for preparatively isolating high-purity 1,1-diphenyl-2-picrylhydrazyl inhibitors from extracts of Saxifraga sinomontana and could be efficiently utilized for rapidly isolating 1,1-diphenyl-2-picrylhydrazyl inhibitors from other natural products.
关键词	OXIDATIVE STRESS FREE-RADICALS ANTIOXIDANTS CONSTITUENTS EXTRACTS ACID GLUCOSIDES GLYCOSIDES LEAVES
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000724948800001
来源期刊	RSC ADVANCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254503
作者单位	[Liu, Chuang; Wang, Qilan] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China; [Ji, Tengfei] Chinese Acad Med Sci, Inst Mat Med, Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Medic, Beijing 100050, Peoples R China; [Ma, Jianbin; Dawa, Yangzom] Qinghai Normal Univ, Coll Life Sci, Qinghai Prov Key Lab Tibet Plateau Biodivers For, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Dang, Jun,Ma, Jianbin,Dawa, Yangzom,et al. Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography[J]. 中国科学院西北生态环境资源研究院,2021,11(61).
APA	Dang, Jun,Ma, Jianbin,Dawa, Yangzom,Liu, Chuang,Ji, Tengfei,&Wang, Qilan.(2021).Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography.RSC ADVANCES,11(61).
MLA	Dang, Jun,et al."Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography".RSC ADVANCES 11.61(2021).