气候变化领域集成服务门户(CCPortal): Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages

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DOI	10.3390/genes12030459
	Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages
	Zhao, Zeying; Zhou, Hanwen; Nie, Zhongnan; Wang, Xuekui; Luo, Biaobiao; Yi, Zhijie; Li, Xinghua; Hu, Xuebo; Yang, Tewu
通讯作者	Yang, TW (通讯作者)，Huazhong Agr Univ, Coll Plant Sci & Technol, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Peoples R China.
发表日期	2021
EISSN	2073-4425
卷号	12 期号:3
英文摘要	Anemone flaccida Fr. Schmidt is a traditional medicinal herb in southwestern China and has multiple pharmacological effects on bruise injuries and rheumatoid arthritis (RA). A new drug with a good curative effect on RA has recently been developed from the extract of A. flaccida rhizomes, of which the main medicinal ingredients are triterpenoid saponins. Due to excessive exploitation, the wild population has been scarce and endangered in a few of its natural habitats and research on the cultivation of the plant commenced. Studies on the gene expressions related to the biosynthesis of triterpenoid saponins are not only helpful for understanding the effects of environmental factors on the medicinal ingredient accumulations but also necessary for monitoring the herb quality of the cultivated plants. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) as a sensitive and powerful technique has been widely used to detect gene expression across tissues in plants at different stages; however, its accuracy and reliability depend largely on the reference gene selection. In this study, the expressions of 10 candidate reference genes were evaluated in various organs of the wild and cultivated plants at different stages, using the algorithms of geNorm, NormFinder and BestKeeper, respectively. The purpose of this study was to identify the suitable reference genes for RT-qPCR detection in A. flaccida. The results showed that two reference genes were sufficient for RT-qPCR data normalization in A. flaccida. PUBQ and ETIF1a can be used as suitable reference genes in most organs at various stages because of their expression stabilitywhereas the PUBQ and EF1 Alpha genes were desirable in the rhizomes of the plant at the vegetative stage.
英文关键词	Anemone flaccida Fr; Schmidt; reference gene; data normalization; RT-qPCR; medicinal herb
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000633662800001
来源期刊	GENES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254500
作者单位	[Zhao, Zeying; Zhou, Hanwen; Wang, Xuekui; Luo, Biaobiao; Yi, Zhijie; Li, Xinghua; Hu, Xuebo; Yang, Tewu] Huazhong Agr Univ, Coll Plant Sci & Technol, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Peoples R China; [Zhao, Zeying] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Lanzhou 730030, Peoples R China; [Zhao, Zeying] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Nie, Zhongnan] Dept Jobs Precincts & Reg, Private Bag 105, Hamilton, Vic 3300, Australia
推荐引用方式 GB/T 7714	Zhao, Zeying,Zhou, Hanwen,Nie, Zhongnan,et al. Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages[J]. 中国科学院西北生态环境资源研究院,2021,12(3).
APA	Zhao, Zeying.,Zhou, Hanwen.,Nie, Zhongnan.,Wang, Xuekui.,Luo, Biaobiao.,...&Yang, Tewu.(2021).Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages.GENES,12(3).
MLA	Zhao, Zeying,et al."Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages".GENES 12.3(2021).