气候变化领域集成服务门户(CCPortal): Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro

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DOI	10.1039/d1ra07273k
	Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro
	Yuan, Chen; Dang, Jun; Han, Yu; Liu, Chuang; Yu, Song; Lv, Yue; Cui, Yunbin; Wang, Zhenhua; Li, Gang
通讯作者	Li, G (通讯作者)，Yantai Univ, Coll Life Sci, Ctr Mitochondria & Hlth Aging, Yantai 264005, Peoples R China.
发表日期	2022
EISSN	2046-2069
起始页码	5031
结束页码	5041
卷号	12 期号:8
英文摘要	Dianthus superbus is a traditional Chinese medicine that is commonly utilized as a treatment for inflammation, pain, and immunological conditions. In this study, an anti-inflammatory maltol glycoside derived from Dianthus superbus was isolated for the first time via medium and high-pressure liquid chromatography, and at the same time, the in vitro anti-inflammatory activity of this maltol glycoside was preliminarily explored. Initially, crude samples of Dianthus superbus were preprocessed via MCI GEL (R) CHP20P and Spherical C18 medium-pressure chromatography, under the guidance of evaluation of in vitro anti-inflammatory activity. Fr44 was found to be the target fraction, and it was further isolated via two-dimensional reversed-phase/hydrophilic interaction liquid chromatography, yielding > 95% pure and was identified as tunicoside B. MTT assay, nitric oxide and nitric oxide synthase were used to evaluate the effects of tunicoside B on murine macrophage Raw264.7 by nitric oxide synthase assay kit, molecular docking, and western blotting. The results showed that tunicoside B did not affect the viability of cells and exhibited significant anti-inflammatory activity. As far as we know, this is the first report of tunicoside B from Dianthus superbus and the first study on the anti-inflammatory activity of tunicoside B. More importantly, the approach established in this study is expected to provide a theoretical basis for the separation and pharmacological activity study of maltol glycosides from other natural products.
关键词	RAW 264.7 LIQUID-CHROMATOGRAPHY PREP-HPLC PURIFICATION EXTRACTION SEPARATION INFLAMMATION ANTIOXIDANT EXPRESSION
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000753032100001
来源期刊	RSC ADVANCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254498
作者单位	[Yuan, Chen; Han, Yu; Lv, Yue; Wang, Zhenhua; Li, Gang] Yantai Univ, Coll Life Sci, Ctr Mitochondria & Hlth Aging, Yantai 264005, Peoples R China; [Dang, Jun; Liu, Chuang] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China; [Yu, Song] Qinghai Univ, Coll Pharm, Xining 810016, Qinghai, Peoples R China; [Cui, Yunbin] Qinghai Normal Univ, Coll Life Sci, Xining 810016, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Chen,Dang, Jun,Han, Yu,et al. Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro[J]. 中国科学院西北生态环境资源研究院,2022,12(8).
APA	Yuan, Chen.,Dang, Jun.,Han, Yu.,Liu, Chuang.,Yu, Song.,...&Li, Gang.(2022).Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro.RSC ADVANCES,12(8).
MLA	Yuan, Chen,et al."Preparative isolation of maltol glycoside from Dianthus superbus and its anti-inflammatory activity in vitro".RSC ADVANCES 12.8(2022).