气候变化领域集成服务门户(CCPortal): Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway

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DOI	10.3389/fmicb.2021.798525
	Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway
	Yue, Liang; Uwaremwe, Constantine; Tian, Yuan; Liu, Yang; Zhao, Xia; Zhou, Qin; Wang, Yun; Zhang, Yubao; Liu, Bailong; Cui, Zengtuan; Dun, Chengchao; Wang, Ruoyu
通讯作者	Wang, RY (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Stress Physiol & Ecol Cold & Arid Reg Gan, Lanzhou, Peoples R China. ; Wang, RY (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Gansu Gaolan Field Sci Observat & Res Stn Agr Eco, Lanzhou, Peoples R China. ; Wang, RY (通讯作者)，Univ Chinese Acad Sci, Beijing, Peoples R China.
发表日期	2022
EISSN	1664-302X
卷号	12
英文摘要	Drought is a major factor limiting the production of the perennial medicinal plant Glycyrrhiza uralensis Fisch. (Fabaceae) in Northwest China. In this study, 1-year-old potted plants were inoculated with the strain Bacillus amyloliquefaciens FZB42, using a gradient of concentrations (CFU), to test for microbe-induced host tolerance to drought condition treatments in a greenhouse experiment. At the concentration of 10(8) CFU ml(-1), FZB42 had significant growth-promoting effect on G. uralensis: the root biomass was 1.52, 0.84, 0.94, and 0.38 times that under normal watering and mild, moderate, and severe drought stress conditions, respectively. Under moderate drought, the positive impact of FZB42 on G. uralensis growth was most pronounced, with both developing axial and lateral roots strongly associated with indoleacetic acid (IAA) accumulation. An untargeted metabolomic analysis and physiological measurements of mature roots revealed that FZB42 improved the antioxidant system of G. uralensis through the accumulation of proline and sucrose, two osmotic adjustment solutes, and by promoting catalase (CAT) activity under moderate drought stress. Furthermore, significantly higher levels of total flavonoids, liquiritin, and glycyrrhizic acid (GA), the pharmacologically active substances of G. uralensis, were found in the roots of inoculated plants after FZB42 inoculation under all imposed drought conditions. The jasmonic acid (JA) content, which is closely related to plant defense responses and secondary metabolites' production, was greatly increased in roots after the bacterial inoculations, indicating that FZB42 activated the JA pathway. Taken together, our results demonstrate that inoculation with FZB42 alleviates the losses in production and pharmacological metabolites of G. uralensis caused by drought via the JA pathway's activation. These results provide a developed prospect of a microbial agent to improve the yield and quality of medical plants in arid and semi-arid regions.
关键词	GROWTH-PROMOTING RHIZOBACTERIA PLANT-GROWTH AZOSPIRILLUM INOCULATION ENDOPHYTIC COLONIZATION METABOLITE ACCUMULATION ANTIOXIDANT ENZYMES SPECTROMETRY DATA METHYL JASMONATE WATER-STRESS MECHANISM
英文关键词	PGPR; Glycyrrhiza uralensis; glycyrrhizic acid (GA); drought; jasmonic acid (JA)
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000778743300001
来源期刊	FRONTIERS IN MICROBIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254355
作者单位	[Yue, Liang; Tian, Yuan; Liu, Yang; Zhao, Xia; Zhou, Qin; Zhang, Yubao; Wang, Ruoyu] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Stress Physiol & Ecol Cold & Arid Reg Gan, Lanzhou, Peoples R China; [Yue, Liang; Tian, Yuan; Liu, Yang; Zhao, Xia; Zhou, Qin; Zhang, Yubao; Wang, Ruoyu] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Gansu Gaolan Field Sci Observat & Res Stn Agr Eco, Lanzhou, Peoples R China; [Yue, Liang; Uwaremwe, Constantine; Tian, Yuan; Liu, Yang; Zhao, Xia; Zhou, Qin; Wang, Yun; Zhang, Yubao; Wang, Ruoyu] Univ Chinese Acad Sci, Beijing, Peoples R China; [Uwaremwe, Constantine] Chinese Acad Sci, CAS Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla, Peoples R China; [Wang, Yun] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Desert & Desertificat, Lanzhou, Peoples R China; [Liu, Bailong] Gansu Inst Drug Control, Lanzhou, Peoples R China; [Cui, Zengtuan] Gen Stn Construction & Protect Cultivated Land &, Lanzhou, Peoples R China;...
推荐引用方式 GB/T 7714	Yue, Liang,Uwaremwe, Constantine,Tian, Yuan,et al. Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway[J]. 中国科学院西北生态环境资源研究院,2022,12.
APA	Yue, Liang.,Uwaremwe, Constantine.,Tian, Yuan.,Liu, Yang.,Zhao, Xia.,...&Wang, Ruoyu.(2022).Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway.FRONTIERS IN MICROBIOLOGY,12.
MLA	Yue, Liang,et al."Bacillus amyloliquefaciens Rescues Glycyrrhizic Acid Loss Under Drought Stress in Glycyrrhiza uralensis by Activating the Jasmonic Acid Pathway".FRONTIERS IN MICROBIOLOGY 12(2022).