气候变化领域集成服务门户(CCPortal): Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography

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DOI	10.1016/j.jchromb.2021.122617
	Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography
	Dang, Jun; Ma, Jianbin; Du, Yurong; Dawa, YangZom; Wang, Qi; Chen, Chengbiao; Wang, Qilan; Tao, Yanduo; Ji, Tengfei
通讯作者	Tao, YD (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China. ; Ji, TF (通讯作者)，Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China.
发表日期	2021
ISSN	1570-0232
EISSN	1873-376X
卷号	1170
英文摘要	In this study, polyamide and MCI GEL? CHP20P were employed as stationary phases in medium pressure chromatography (MPC) for the efficient preparative separation of bergenin from Saxifraga atrata. Ethanol?water, methanol-water, and acetonitrile-water mobile phases all showed good enrichment capacity for bergenin fraction when polyamide was used as a stationary phase. After 5 cycles of polyamide MPC using acetonitrile/water, 1.2 g of bergenin fraction was isolated from 180 g Saxifraga atrata herb. Further purification of this fraction was conducted using MCI GEL? CHP20P styrene-divinylbenzene beads. The bergenin fraction was separated into two fractions, and after three runs of MPC, 714.2 mg of bergenin with purity above 99% was obtained. The results demonstrate that the combination of polyamide and styrene-divinylbenzene MPC can be utilized for preparative isolation of compounds from natural products with high yield and purity.
关键词	LIQUID-CHROMATOGRAPHY SEPARATION
英文关键词	Saxifraga atrata; Polyamide; Micro gel chp20p; Medium pressure chromatography; Bergenin
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000639872900002
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254323
作者单位	[Dang, Jun; Wang, Qilan; Tao, Yanduo] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China; [Ma, Jianbin; Du, Yurong; Dawa, YangZom; Chen, Chengbiao] Qinghai Normal Univ, Coll Life Sci, Qinghai Prov Key Lab Tibet Plateau Biodivers Form, Xining 810008, Qinghai, Peoples R China; [Wang, Qi] Qinghai Nationalities Univ, Coll Pharm, Xining 810007, Qinghai, Peoples R China; [Ji, Tengfei] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Dang, Jun,Ma, Jianbin,Du, Yurong,et al. Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography[J]. 中国科学院西北生态环境资源研究院,2021,1170.
APA	Dang, Jun.,Ma, Jianbin.,Du, Yurong.,Dawa, YangZom.,Wang, Qi.,...&Ji, Tengfei.(2021).Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1170.
MLA	Dang, Jun,et al."Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1170(2021).