气候变化领域集成服务门户(CCPortal): Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay

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DOI	10.1016/j.jep.2021.114785
	Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay
	Wang, Weidong; Jiang, Lei; Zhu, Yunhe; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen
通讯作者	Tao, YD ; Liu, ZG (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China.
发表日期	2022
ISSN	0378-8741
EISSN	1872-7573
卷号	284
英文摘要	Ethnopharmacological relevance: Saussurea obvallata (DC.) Edgew. is a traditional Tibetan medicine used for the treatment of inflammation-related diseases, but the scientific validation was very limited. Aim of the study: This study aimed to rapid screen and targeted isolate cyclooxygenase-2 (COX-2) inhibitors from S. obvallata extract. Materials and methods: An efficient ligand-fishing method based on affinity solid phase extraction (A-SPE) combining with HPLC was developed. The identified COX-2 inhibitors were separated using preparative liquid chromatography. In vitro COX-2 inhibition assays were employed to confirm the inhibitory activities of the isolated compounds. In addition, the effect of the isolated compounds on the production of prostaglandin E2 (PGE2) and the expression of COX-2 in LPS-induced RAW 264.7 were evaluated. Results: A total of four phenylpropanoids, isolariciresinol, syringaresinol, pinoresinol and balanophonin were targeted isolated as COX-2 inhibitors with IC50 values of 36.4 +/- 2.6 mu M, 23.1 +/- 1.8 mu M, 3.6 +/- 0.3 mu M and 12.1 +/- 0.9 mu M, respectively. The isolated compounds significantly inhibited LPS-induced NO production in a dose-dependent manner. And, the results of the inhibitory effect on the release of PGE(2) and the expression of COX-2 in LPS-induced macrophages were consistent with A-SPE analysis. Conclusion: The present work demonstrated that the developed A-SPE-HPLC method could successfully targeted isolated COX-2 inhibitors from S. obvallata extract. And, the isolation results indicated that the therapeutic effect of S. obvallata on inflammation-related diseases was partly based on the COX-2 active ingredients.
关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY CHINESE MEDICINES SEPARATION SYSTEM IMMOBILIZATION IDENTIFICATION MICROSPHERES CONSTITUENTS COMPONENTS
英文关键词	Saussurea obvallata; Inflammation; Affinity solid phase extraction; Cyclooxygenase-2 inhibitors; Phenylpropanoids
语种	英语
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000718038500004
来源期刊	JOURNAL OF ETHNOPHARMACOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254261
作者单位	[Wang, Weidong; Jiang, Lei; Zhu, Yunhe; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China; [Wang, Weidong; Jiang, Lei; Zhu, Yunhe; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen] Qinghai Prov Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China; [Wang, Weidong; Zhu, Yunhe] Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weidong,Jiang, Lei,Zhu, Yunhe,et al. Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay[J]. 中国科学院西北生态环境资源研究院,2022,284.
APA	Wang, Weidong,Jiang, Lei,Zhu, Yunhe,Mei, Lijuan,Tao, Yanduo,&Liu, Zenggen.(2022).Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay.JOURNAL OF ETHNOPHARMACOLOGY,284.
MLA	Wang, Weidong,et al."Bioactivity-guided isolation of cyclooxygenase-2 inhibitors from Saussurea obvallata (DC.) Edgew. Using affinity solid phase extraction assay".JOURNAL OF ETHNOPHARMACOLOGY 284(2022).