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DOI	10.1080/01496395.2021.1995425
	Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography
	Chen, Tao; Liu, Yongling; Wang, Shuo; Wei, Yangfei; Song, Zhibo; Yan, Shuping; Shen, Cheng; Li, Hongmei; Li, Peipei; Li, Yulin
通讯作者	Li, YL (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China.
发表日期	2020
ISSN	0149-6395
EISSN	1520-5754
英文摘要	In this study, eight phenolic compounds were obtained from the over-ground parts of the Aconitum pendulum (A. pendulum) Busch through an efficient high-speed counter-current chromatography (HSCCC?method. First, the column chromatography for macroporous resin was used for the treatment of crude extract of over-ground parts of A. pendulum Busch. And then, 30% ethanol elution fraction was transferred to HSCCC for separation using the classic ethyl acetate, n-butanol and water system with the solvent ratio of 7:3:10. To improve parting efficiency, a mode of repeated injection was developed. Two groups of compounds existing in one HPLC peak were effectively separated by HSCCC. The results revealed that HSCCC could effectively isolate compounds with the same or similar polarity. Preparative HPLC was used to perform further purification, and then eight phenolic compounds were separated from the over-ground parts of the A. pendulum Busch for the first time. The current investigation successfully provides a reference for phytochemical research and, comprehensive development and utilization of A. pendulum Busch in the future. Furthermore, HSCCC efficiently separated compounds with similar polarity and its combination with preparative HPLC could play a great role in the separation of natural materials.
关键词	PREPARATIVE SEPARATION STATIONARY-PHASE HPLC SEPARATION PURIFICATION COLUMN ANTIOXIDANT GLYCOSIDES RESOLUTION ISOMERS GALLATE
英文关键词	Aconitum pendulum busch; first separation; counter-current chromatography; repeated injection; phenolic compounds
语种	英语
WOS研究方向	Chemistry ; Engineering
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000711778100001
来源期刊	SEPARATION SCIENCE AND TECHNOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254209
作者单位	[Chen, Tao; Wang, Shuo; Wei, Yangfei; Song, Zhibo; Yan, Shuping; Shen, Cheng; Li, Hongmei; Li, Peipei; Li, Yulin] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China; [Chen, Tao; Wei, Yangfei; Song, Zhibo; Yan, Shuping; Shen, Cheng; Li, Hongmei; Li, Peipei] Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China; [Liu, Yongling] Guiyang Univ, Food & Pharmaceut Engn Inst, Guiyang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Tao,Liu, Yongling,Wang, Shuo,et al. Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography[J]. 中国科学院西北生态环境资源研究院,2020.
APA	Chen, Tao.,Liu, Yongling.,Wang, Shuo.,Wei, Yangfei.,Song, Zhibo.,...&Li, Yulin.(2020).Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	Chen, Tao,et al."Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2020).