气候变化领域集成服务门户(CCPortal): Hypobaric hypoxia exposure alters transcriptome in mouse testis and impairs spermatogenesis in offspring

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DOI	10.1016/j.gene.2022.146390
	Hypobaric hypoxia exposure alters transcriptome in mouse testis and impairs spermatogenesis in offspring
	Li, Shuang; Yang, Qi-En
通讯作者	Yang, QE (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China.
发表日期	2022
ISSN	0378-1119
EISSN	1879-0038
卷号	823
英文摘要	Male fertility relies on continual and robust spermatogenesis. Environmental hypoxia adversely affects reproductive health in humans and animal studies provide compelling evidences that hypoxia impairs spermatogenesis in directly exposed individuals. However, a detail examination of hypoxia induced changes in testicular gene expression is still lacking and spermatogenesis in offspring of hypoxia exposed animals of awaits investigation. In this study, a hypobaric hypoxic chamber was used to simulate hypoxic conditions in mice and effects of hypoxia on spermatogenesis, fertility and testicular gene expression were evaluated. The results showed that hypoxia exposure reduced the number of undifferentiated spermatogonia but did not change the regenerative capacity of spermatogonial stem cells (SSCs) after transplantation. Hypoxia significantly increased the percent of abnormal sperm and these defects were recovered 2 months after returning to the normoxia. Transcriptome analysis of testicular tissues from control and hypoxia treated animals revealed that 766 genes were up-regulated and 965 genes were down-regulated. Surprisingly, expressions of genes that regulate epigenetic modifications were altered, indicating hypoxia-induced damage to spermatogenesis may be intergenerational. Indeed, animals that were sired by hypoxia exposed males exhibited impaired spermatogenesis. Together, these findings suggest that hypoxia exposure alters testicular gene expression and causes long-lasting damage to spermatogenesis.
关键词	SERTOLI-CELLS HIGH-ALTITUDE
英文关键词	Hypoxia; Spermatogenesis; Transcriptome; Intergenerational; Sperm; Epigenetics
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000777837500003
来源期刊	GENE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254156
作者单位	[Li, Shuang; Yang, Qi-En] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China; [Li, Shuang; Yang, Qi-En] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang, Qi-En] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Anim Ecol Genom, Xining 810001, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Li, Shuang,Yang, Qi-En. Hypobaric hypoxia exposure alters transcriptome in mouse testis and impairs spermatogenesis in offspring[J]. 中国科学院西北生态环境资源研究院,2022,823.
APA	Li, Shuang,&Yang, Qi-En.(2022).Hypobaric hypoxia exposure alters transcriptome in mouse testis and impairs spermatogenesis in offspring.GENE,823.
MLA	Li, Shuang,et al."Hypobaric hypoxia exposure alters transcriptome in mouse testis and impairs spermatogenesis in offspring".GENE 823(2022).