气候变化领域集成服务门户(CCPortal): Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry

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DOI	10.1016/j.jchromb.2021.122919
	Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry
	Dong, Qi; Hu, Na; Yue, Huilan; Wang, Honglun; Ku, Jinliang
通讯作者	Wang, HL (通讯作者)，Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China. ; Wang, HL (通讯作者)，Northwest Inst Plateau Biol, CAS Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China.
发表日期	2021
ISSN	1570-0232
EISSN	1873-376X
卷号	1181
英文摘要	Extracts from the bran of Chenopodium quinoa Willd. (QBE) were reported to be active in inhibiting alpha-glycosidase, a promising target for treatment of diabetes mellitus. However, the constituents responsible for the alpha-glucosidase-inhibiting activity of QBE have not been fully characterized. The present study aimed to set up a method for rapid identification of glycosidase inhibiting compounds from the quinoa bran. With surface plasmon resonance (SPR) coupled with liquid chromatography-mass spectrometry (LC-MS), we identified eight flavonoids and ten triterpenoid saponins that may bind to the alpha-glycosidase. Analysis of the interaction kinetics by molecular docking supported their alpha-glucosidase-inhibiting activity and revealed the potential mechanisms for the inhibitory effects. In summary, this study established a SPR and LC-MS-based method for rapid in vitro screening of alpha-glucosidase inhibitors and suggested the quinoa bran a potential natural source of alpha-glucosidase inhibitors.
关键词	FLAVONOID GLYCOSIDES MOLECULAR DOCKING BINDING ANTIOXIDANT CONSTITUENTS BIOSENSOR MECHANISM SAPONINS KINETICS AMYLASE
英文关键词	Chenopodium quinoa Willd; alpha-glucosidase inhibitors; Surface plasmon resonance; Liquid chromatography-mass spectrometry; Molecular docking analysis
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000703594000003
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254140
作者单位	[Dong, Qi; Hu, Na; Yue, Huilan; Wang, Honglun] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China; [Dong, Qi; Hu, Na; Yue, Huilan; Wang, Honglun] Northwest Inst Plateau Biol, CAS Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China; [Dong, Qi] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ku, Jinliang] Beijing Tongrentang Hlth Pharmaceut Qinghai Co Lt, Xining 817000, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Dong, Qi,Hu, Na,Yue, Huilan,et al. Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry[J]. 中国科学院西北生态环境资源研究院,2021,1181.
APA	Dong, Qi,Hu, Na,Yue, Huilan,Wang, Honglun,&Ku, Jinliang.(2021).Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1181.
MLA	Dong, Qi,et al."Identification of alpha-glucosidase inhibitors from the bran of Chenopodium quinoa Willd. by surface plasmon resonance coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1181(2021).