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DOI	10.3897/compcytogen.v15.i4.71525
	The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes
	Tao, Xiaoyan; Liu, Bo; Dou, Quanwen
通讯作者	Dou, QW (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China. ; Dou, QW (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Crop Mol Breeding, Xining, Qinghai, Peoples R China.
发表日期	2021
ISSN	1993-0771
EISSN	1993-078X
起始页码	375
结束页码	392
卷号	15 期号:4
英文摘要	Kengyilia hirsuta (Keng, 1959) J. L. Yang, C. Yen et B. R. Baum, 1992, a perennial hexaploidy species, is a wild relative species to wheat with great potential for wheat improvement and domestication. The genome structure and cross-species homoeology of K. hirsuta chromosomes with wheat were assayed using 14 single gene probes covering all seven homoeologous groups, and four repetitive sequence probes 45S rDNA, 5S rDNA, pAs1, and (AAG)(10) by FISH. Each chromosome of K. hirsuta was well characterized by homoeological determination and repeats distribution patterns. The synteny of chromosomes was strongly conserved in the St genome, whereas synteny of the Y and P genomes was more distorted. The collinearity of 1Y, 2Y, 3Y and 7Y might be interrupted in the Y genome. A new 5S rDNA site on 2Y might be translocated from 1Y. The short arm of 3Y might involve translocated segments from 7Y. The 7 Y was identified as involving a pericentric inversion. A reciprocal translocation between 2P and 4P, and tentative structural aberrations in the subtelomeric region of 1PL and 4PL, were observed in the P genome. Chromosome polymorphisms, which were mostly characterized by repeats amplification and deletion, varied between chromosomes, genomes, and different populations. However, two translocations involving a P genome segmental in 3YL and a non-Robertsonial reciprocal translocation between 4Y and 3P were identified in two independent populations. Moreover, the proportion of heterozygous karyotypes reached almost 35% in all materials, and almost 80% in the specific population. These results provide new insights into the genome organization of K. hirsuta and will facilitate genome dissection and germplasm utilization of this species.
关键词	IN-SITU HYBRIDIZATION RNA MULTIGENE FAMILY GENOME ANALYSIS CHROMOSOMAL REARRANGEMENTS REPETITIVE DNA ELYMUS WHEAT POACEAE TRANSLOCATIONS IDENTIFICATION
英文关键词	Homoeology; karyotype; Kengyilia hirsuta; single-gene FISH
语种	英语
WOS研究方向	Plant Sciences ; Genetics & Heredity ; Zoology
WOS类目	Plant Sciences ; Genetics & Heredity ; Zoology
WOS记录号	WOS:000717672500002
来源期刊	COMPARATIVE CYTOGENETICS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254079
作者单位	[Tao, Xiaoyan; Liu, Bo; Dou, Quanwen] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China; [Dou, Quanwen] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Crop Mol Breeding, Xining, Qinghai, Peoples R China; [Tao, Xiaoyan; Liu, Bo] Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Tao, Xiaoyan,Liu, Bo,Dou, Quanwen. The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes[J]. 中国科学院西北生态环境资源研究院,2021,15(4).
APA	Tao, Xiaoyan,Liu, Bo,&Dou, Quanwen.(2021).The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes.COMPARATIVE CYTOGENETICS,15(4).
MLA	Tao, Xiaoyan,et al."The Kengyilia hirsuta karyotype polymorphisms as revealed by FISH with tandem repeats and single-gene probes".COMPARATIVE CYTOGENETICS 15.4(2021).