气候变化领域集成服务门户(CCPortal): Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl detection

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DOI	10.1016/j.chroma.2020.461690
	Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl detection
	Dawa, Yangzom; Du, Yurong; Wang, Qi; Chen, Chengbiao; Zou, Denglang; Qi, Desheng; Ma, Jianbin; Dang, Jun
通讯作者	Ma, JB (通讯作者)，Qinghai Normal Univ, Coll Life Sci, Qinghai Prov Key Lab Tibet Plateau Biodivers Form, Xining 810008, Peoples R China. ; Dang, J (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China.
发表日期	2021
ISSN	0021-9673
EISSN	1873-3778
卷号	1635
英文摘要	Traditional Tibetan medicine (TTM) is a valuable source of novel therapeutic lead molecules inspired by natural products (NPs). The health benefits of Saxifraga atrata are well documented in TTM, but reports on its chemical composition are limited, most likely due to the complicated purification process. Herein, target separation and identification of 4 main radical scavenging compounds from the methanolic extract of S. atrata was were performed using mediumand high-pressure liquid chromatography coupled with online HPLC-DPPH detection. The sample was pretreated using medium pressure liquid chromatography with MCI GEL (R) CHP20P styrene-divinylbenzene beads as a stationary phase, yielding 1.4 g of the target DPPH inhibitors (Fr4, 11.9% recovery). The compounds were further purified and isolated using HPLC on RP-C18 (ReproSil-Pur C18 AQ) followed by HILIC (Click XIon) column separation, resulting in 2.8 mg of fraction Fr4-1-1, 6.8 mg of fraction Fr4-2, 244.9 mg of the Fr4-3-1 sample, and 38.3 mg of Fr4-4-1. The structure and purity of the target compounds were determined, and four compounds (ethyl gallate, 11-O-galloylbergenin, rutin and isoquercitrin) were isolated with > 95% purity. The developed methodology is efficient for targeted isolation of high-purity radical scavengers from NP extracts and could be used for rapid identification and isolation of DPPH inhibitors from various NPs. (C) 2020 Elsevier B.V. All rights reserved.
关键词	RADICAL SCAVENGING COMPOUNDS DPPH SCREENING METHOD ANTIOXIDANT L.FLAVONOIDS GLYCOSIDES EXTRACTS LEAVES OIL
英文关键词	Saxifraga atrata; Online HPLC-DPPH assay; Medium-pressure liquid chromatography; High-pressure liquid chromatography; DPPH inhibitors
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000604226500013
来源期刊	JOURNAL OF CHROMATOGRAPHY A
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254056
作者单位	[Dawa, Yangzom; Du, Yurong; Chen, Chengbiao; Zou, Denglang; Qi, Desheng; Ma, Jianbin] Qinghai Normal Univ, Coll Life Sci, Qinghai Prov Key Lab Tibet Plateau Biodivers Form, Xining 810008, Peoples R China; [Dang, Jun] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China; [Wang, Qi] Qinghai Nationalities Univ, Coll Pharm, Xining, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Dawa, Yangzom,Du, Yurong,Wang, Qi,et al. Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl detection[J]. 中国科学院西北生态环境资源研究院,2021,1635.
APA	Dawa, Yangzom.,Du, Yurong.,Wang, Qi.,Chen, Chengbiao.,Zou, Denglang.,...&Dang, Jun.(2021).Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl detection.JOURNAL OF CHROMATOGRAPHY A,1635.
MLA	Dawa, Yangzom,et al."Targeted isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Saxifraga atrata using medium- and high- pressure liquid chromatography combined with online high performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl detection".JOURNAL OF CHROMATOGRAPHY A 1635(2021).