气候变化领域集成服务门户(CCPortal): Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid

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DOI	10.3390/ijms222111424
	Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid
	Liu, Bo; Wang, Sui; Tao, Xiaoyan; Liu, Caixia; Qu, Guanzheng; Dou, Quanwen
通讯作者	Dou, QW (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China. ; Qu, GZ (通讯作者)，Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China. ; Dou, QW (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Crop Mol Breeding Qinghai Prov, Xining 810008, Peoples R China.
发表日期	2021
EISSN	1422-0067
卷号	22 期号:21
英文摘要	The molecular karyotype could represent the basic genetic make-up in a cell nucleus of an organism or species. A doubled haploid (DH) is a genotype formed from the chromosome doubling of haploid cells. In the present study, molecular karyotype analysis of the poplar hybrid Populus simonii x P. nigra (P. xiaohei) and the derived doubled haploids was carried out with labeled telomeres, rDNA, and two newly repetitive sequences as probes by fluorescence in situ hybridization (FISH). The tandem repeats, pPC349_XHY and pPD284_XHY, with high-sequence homology were used, and the results showed that they presented the colocalized distribution signal in chromosomes. For P. xiaohei, pPD284_XHY produced hybridizations in chromosomes 1, 5, 8, and 9 in the hybrid. The combination of pPD284_XHY, 45S rDNA, and 5S rDNA distinctly distinguished six pairs of chromosomes, and the three pairs of chromosomes showed a significant difference in the hybridization between homologous chromosomes. The repeat probes used produced similar FISH hybridizations in the DH; nevertheless, pPD284_XHY generated an additional hybridization site in the telomere region of chromosome 14. Moreover, two pairs of chromosomes showed differential hybridization distributions between homologous chromosomes. Comparisons of the distinguished chromosomes between hybrid and DH poplar showed that three pairs of chromosomes in the DH presented hybridization patterns that varied from those of the hybrid. The No. 8 chromosome in DH and one of the homologous chromosomes in P. xiaohei shared highly similar FISH patterns, which suggested the possibility of intact or mostly partial transfer of the chromosome between the hybrid and DH. Our study will contribute to understanding the genetic mechanism of chromosomal variation in P. xiaohei and derived DH plants.
关键词	IN-SITU HYBRIDIZATION RIBOSOMAL DNA PLANT GENOME FISH CHROMOSOMES SEQUENCE LOCI GISH
英文关键词	Populus; Populus simonii x P. nigra; doubled haploid; repetitive sequence; karyotype; FISH
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000722332200001
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254029
作者单位	[Liu, Bo; Tao, Xiaoyan; Dou, Quanwen] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China; [Wang, Sui; Liu, Caixia; Qu, Guanzheng] Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China; [Wang, Sui] Northeast Agr Univ, Minist Educ China, Key Lab Soybean Biol, Harbin 150030, Peoples R China; [Tao, Xiaoyan] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China; [Dou, Quanwen] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Crop Mol Breeding Qinghai Prov, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bo,Wang, Sui,Tao, Xiaoyan,et al. Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid[J]. 中国科学院西北生态环境资源研究院,2021,22(21).
APA	Liu, Bo,Wang, Sui,Tao, Xiaoyan,Liu, Caixia,Qu, Guanzheng,&Dou, Quanwen.(2021).Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(21).
MLA	Liu, Bo,et al."Molecular Karyotyping on Populus simonii x P. nigra and the Derived Doubled Haploid".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.21(2021).