气候变化领域集成服务门户(CCPortal): Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau

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DOI	10.1016/j.jep.2021.114068
	Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau
	Zhou, Dangwei; Lv, Dongjin; Zhang, Hui; Cheng, Tingfeng; Wang, Huan; Lin, Pengcheng; Shi, Shengbo; Chen, Shilong; Shen, Jianwei
通讯作者	Zhou, DW (通讯作者)，Qinghai Nationalities Univ, Key Lab Tibet Plateau Phytochem Qinghai Prov, Xining 810007, Qinghai, Peoples R China.
发表日期	2021
ISSN	0378-8741
EISSN	1872-7573
卷号	280
英文摘要	Ethnobotanical relevance: Gentiana straminea Maxim. is a well-known Tibetan traditional herb, which has been used to treat rheumatic arthritis, iceteric hepatitis, and other diseases for thousands years. However, there is still lack of comprehensive active constituents profiling of this species throughout the Qinghai-Tibet Plateau (QTP). Aim of study: This study was designed to provide a comprehensive quality map of G.straminea germplasm based on twelve active constituents (loganic acid, gentiopicroside, swertiamarin, sweroside, 6-O-beta-D-glucosylgentio-picroside, oleanic acid, morroniside, trilobatin, isoorientin, isovite, Shanzhisidemethylester and quercetin) on the QTP. Materials and methods: G.straminea root samples collected throughout QTP in the flowering period were analyzed by the LC-MS/MS. Statistics analysis methods PCA, clustering and ecological regions analysis for G.straminea constituents differentiation was demonstrated. Results: The active constituents varied greatly across the QTP; the majority of constituents were secoiridoid derivatives, with gentiopicroside being the most abundant compound. Most constituents were significantly affected by the latitudes and altitudes other than longitudes. PCA and hierarchical clustering analysis showed that all samples could be separated into six distinct groups, and 15 populations showed the highest constituent abundances. Further, geographical region analysis showed that the highest quality populations mainly located near the source region of Yellow River, especially in the Qinghai and Sichuan areas. Additionally, correlation analysis showed that there were relationships among genetiopicroside, loganic acid, and other compounds, which might be related to the enzymatic pathways involved in the metabolism of these constituents. Conclusion: LC-MS/MS method allowed separation of quality profiling of G.straminea on the QTP, 15 populations showed the highest constituent abundances. In six geographical groups, the highest quality populations mainly located near the source region of Yellow River, especially in the Qinghai and Sichuan areas, which may be due to the climate caused by the westerlies and Indian Ocean monsoons in the QTP.
关键词	LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY SECT. CRUCIATA IRIDOIDS SECOIRIDOIDS CONSTITUENTS MEDICINE AUTHENTICATION XANTHONES PROGRESS
英文关键词	Gentiana straminea Maxim; Active compounds; LC-MS; MS; Qinghai-Tibetan plateau; Source region of yellow river
语种	英语
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000704751500002
来源期刊	JOURNAL OF ETHNOPHARMACOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253989
作者单位	[Zhou, Dangwei; Lin, Pengcheng] Qinghai Nationalities Univ, Key Lab Tibet Plateau Phytochem Qinghai Prov, Xining 810007, Qinghai, Peoples R China; [Zhou, Dangwei; Cheng, Tingfeng; Shi, Shengbo; Chen, Shilong] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China; [Lv, Dongjin; Zhang, Hui; Shen, Jianwei] Qinghai Ctr Dis Prevent & Control, Xining 810007, Qinghai, Peoples R China; [Wang, Huan] Chinese Acad Sci, Tibetan Med Ctr, Northwest Inst Plateau Biol, Xining 810008, Qinghai, Peoples R China; [Zhou, Dangwei; Cheng, Tingfeng; Chen, Shilong] Univ Chinese Acad Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Dangwei,Lv, Dongjin,Zhang, Hui,et al. Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau[J]. 中国科学院西北生态环境资源研究院,2021,280.
APA	Zhou, Dangwei.,Lv, Dongjin.,Zhang, Hui.,Cheng, Tingfeng.,Wang, Huan.,...&Shen, Jianwei.(2021).Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau.JOURNAL OF ETHNOPHARMACOLOGY,280.
MLA	Zhou, Dangwei,et al."Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau".JOURNAL OF ETHNOPHARMACOLOGY 280(2021).