气候变化领域集成服务门户(CCPortal): Effects of small-molecule compounds on fibroblast properties in golden snub-nosed monkey (Rhinopithecus roxellana)

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DOI	10.1111/jmp.12549
	Effects of small-molecule compounds on fibroblast properties in golden snub-nosed monkey (Rhinopithecus roxellana)
	Wang, Juanjuan; Liu, Xin; Yang, Jing; Guo, Hanxing; Li, Jingjing; Huo, Lihui; Zhao, Haitao; Wang, Xiaowei; Yan, Xingrong; Li, Baoguo; Sun, Yu
通讯作者	Wang, XW (通讯作者)，Shaanxi Inst Zool, Northwest Inst Endangered Zool Species, Xian 710032, Peoples R China. ; Yan, XR ; Li, BG (通讯作者)，Northwest Univ, Coll Life Sci, Xian 710069, Peoples R China.
发表日期	2021
ISSN	0047-2565
EISSN	1600-0684
起始页码	323
结束页码	331
卷号	50 期号:6
英文摘要	Background Golden snub-nosed monkey (Rhinopithecus roxellana) is an endangered primate species, whose molecular material for conservation purposes has not yet been maintained. Although small-molecule compounds (SMCs) have been reported to improve induced pluripotent stem cells (iPSCs), their efficiency in the interspecies-transferred nucleus is still unknown. Methods We thus used the fibroblasts from the golden snub-nosed monkey treated with SMC as donor cells, injected into the enucleated oocytes of goats, to test such efficiency. Gene expression profiles in the cell-constructed embryos with and without SMCs were compared by qPCR. Results The results show that cell morphology undergoes remarkable changes (volume is smaller than normal cells, and many black spots in the cytoplasm were found); pluripotent genes (Oct4, Sox2, and Nanog) significantly increased with SMC treatment. Conclusions This study demonstrates that SMCs alter the properties of donor cells and promote the expression of pluripotent genes in hybrid embryos.
关键词	NUCLEAR TRANSFER STEM-CELLS PLURIPOTENCY FATE DNA
英文关键词	embryo; goat; iSCNT; Sichuan golden snub-nosed monkey; small-molecule compounds
语种	英语
WOS研究方向	Veterinary Sciences ; Zoology
WOS类目	Veterinary Sciences ; Zoology
WOS记录号	WOS:000708276600001
来源期刊	JOURNAL OF MEDICAL PRIMATOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253960
作者单位	[Wang, Juanjuan; Liu, Xin; Yang, Jing; Guo, Hanxing; Huo, Lihui; Yan, Xingrong; Li, Baoguo; Sun, Yu] Northwest Univ, Coll Life Sci, Key Lab Resource Biol & Biotechnol Western China, Minist Educ,Shaanxi Key Lab Anim Conservat, Xian, Peoples R China; [Li, Jingjing] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Xian, Peoples R China; [Zhao, Haitao; Wang, Xiaowei] Shaanxi Inst Zool, Northwest Inst Endangered Zool Species, Xian 710032, Peoples R China; [Li, Baoguo] Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Wang, Juanjuan,Liu, Xin,Yang, Jing,et al. Effects of small-molecule compounds on fibroblast properties in golden snub-nosed monkey (Rhinopithecus roxellana)[J]. 中国科学院西北生态环境资源研究院,2021,50(6).
APA	Wang, Juanjuan.,Liu, Xin.,Yang, Jing.,Guo, Hanxing.,Li, Jingjing.,...&Sun, Yu.(2021).Effects of small-molecule compounds on fibroblast properties in golden snub-nosed monkey (Rhinopithecus roxellana).JOURNAL OF MEDICAL PRIMATOLOGY,50(6).
MLA	Wang, Juanjuan,et al."Effects of small-molecule compounds on fibroblast properties in golden snub-nosed monkey (Rhinopithecus roxellana)".JOURNAL OF MEDICAL PRIMATOLOGY 50.6(2021).