气候变化领域集成服务门户(CCPortal): Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu

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DOI	10.1080/13102818.2020.1868332
	Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu
	Cao, Dong; Ye, Fahui; Zhao, Caixia; Shen, Jicheng; Liu, Ruijuan; Zhang, Bo; Shen, Yuhu; Zhang, Huaigang; Liu, Baolong; Chen, Wenjie
通讯作者	Liu, BL ; Chen, WJ (通讯作者)，Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Qinghai, Peoples R China.
发表日期	2021
ISSN	1310-2818
EISSN	1314-3530
起始页码	276
结束页码	282
卷号	35 期号:1
英文摘要	Avenin-like storage proteins affect the rheological properties and processing quality of common wheat. Triticum urartu, the A-genome donor of polyploid wheat, shows abundant variation in its glutenin subunits. In this study, the variability in avenin-like b genes was evaluated in 74 T. urartu accessions. High levels of polymorphism were demonstrated in the analyzed sequence, resulting in the discovery of 18 novel TuALPb7A alleles (TuALPb7A-A-TuALPb7A-R). There were nucleotide sequence differences in 18 locations, which, due to synonymous mutations, resulted in amino acid changes. The polymorphic sites were located in the N-terminus, repetitive region (Left), repetitive region (Right) and C-terminus domains. The position of some cysteine residues was different in T. urartu and Aegilops tauschii accessions. Phylogenetic analysis divided the avenin-like b (ALPb) genes into two groups. The ALPb-7A alleles, containing 18 TuALPb7As, were distributed in the first group, whereas the ALPb-4A alleles were present in another. Avenin-like proteins encoded by four alleles (TuALPb7A-G, TuALPb7A-O, TuALPb7A-K and TuALPb7A-C) were expressed on a large scale and purified to estimate the effect on dough mixing properties. Mixograph testing demonstrated that these proteins could improve the functional properties of dough, including the mixing time, peak dough resistance and breakdown in resistance. The avenin-like b genes hold rich genetic alleles in T. urartu, and can improve the functional properties of dough. The new favourable alleles discovered will provide breeding materials and a theoretical basis for wheat quality improvement.
关键词	WEIGHT GLUTENIN SUBUNIT HETEROLOGOUS EXPRESSION WHEAT AEGILOPS DOUGHS DURUM HMW
英文关键词	Triticum urartu; avenin-like b gene; TuALPb7A; genetic diversity; dough properties
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000605731200001
来源期刊	BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253929
作者单位	[Cao, Dong; Ye, Fahui; Zhao, Caixia; Shen, Jicheng; Liu, Ruijuan; Zhang, Bo; Shen, Yuhu; Zhang, Huaigang; Liu, Baolong; Chen, Wenjie] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Prov Key Lab Crop Mol Breeding, Xining, Qinghai, Peoples R China; [Cao, Dong; Ye, Fahui; Zhao, Caixia; Shen, Jicheng; Liu, Ruijuan; Zhang, Bo; Shen, Yuhu; Zhang, Huaigang; Liu, Baolong; Chen, Wenjie] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai, Peoples R China; [Cao, Dong; Ye, Fahui; Zhao, Caixia; Shen, Jicheng; Liu, Ruijuan; Zhang, Bo; Shen, Yuhu; Zhang, Huaigang; Liu, Baolong; Chen, Wenjie] Chinese Acad Sci, Innovat Acad Seed Design, Lab Wheat Qual Improvement, Beijing, Peoples R China; [Ye, Fahui; Zhao, Caixia; Zhang, Huaigang] Univ Chinese Acad Sci, Sch Life Sci, Dept Genet & Dev Biol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cao, Dong,Ye, Fahui,Zhao, Caixia,et al. Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu[J]. 中国科学院西北生态环境资源研究院,2021,35(1).
APA	Cao, Dong.,Ye, Fahui.,Zhao, Caixia.,Shen, Jicheng.,Liu, Ruijuan.,...&Chen, Wenjie.(2021).Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,35(1).
MLA	Cao, Dong,et al."Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 35.1(2021).