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DOI	10.1002/jssc.202100537
	Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes
	Chen, Tao; Yang, Xue; Wang, Shuo; Shen, Cheng; Li, Hongmei; Wei, Yangfei; Yan, Shuping; Song, Zhibo; Yang, Fengmei; Liu, Yarong; Hai, Ping; Li, Yulin
通讯作者	Li, YL (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China. ; Hai, P (通讯作者)，Qinghai Inst Food & Drug Control, Key Lab Qual Control Tradit Chinese Med Tibetan M, Natl Med Prod Adm, Xining 810000, Peoples R China.
发表日期	2022
ISSN	1615-9306
EISSN	1615-9314
起始页码	468
结束页码	476
卷号	45 期号:2
英文摘要	The separation of compounds with similar polarities is challenging. In the present study, five flavone glycosides, including two groups with similar polarities, were obtained from Dracocephalum tanguticum by three high-speed counter-current chromatography modes, including flow rate conversion mode, recycling mode, and heart-cut mode. With flow rate conversion mode, compounds 3 and 4 with similar polarities and compound 5 were separated by high-speed counter-current chromatography with ethyl acetate/methanol/water (5.0% acetic acid) (8:2:10, v/v) system. The flow rate was controlled as: 1.8 mL/min for 0-160 min, 2.2 mL/min for 160-200 min, and 2.5 mL/min for 200-400 min. However, compounds 1 and 2 with similar polarities were not separated due to the similar distributive properties. Then, a recycling and heart-cut mode were introduced to improve the separation efficiency. The heart-cut mode was introduced in the second and third cycles, and compounds 1 and 2 were well separated in the fourth cycle. Consequently, five flavone glycosides, including two groups with similar polarities were obtained and identified as cosmosiin (1), pedaliin (2), quercetin-3-O-rutinoside (3), pedaliin-6''-acetate (4), and sorbifolin-6-O-beta-glucopyranoside (5). The current strategy provides a reference for separating compounds with similar polarities from a crude sample.
关键词	LIQUID-CHROMATOGRAPHY FLOW-RATE PURIFICATION LEAVES ANTIOXIDANT ENANTIOMERS SAPONINS
英文关键词	counter-current chromatography; flow rate conversion; heart-cutting; recycling; similar polarities
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000718888300001
来源期刊	JOURNAL OF SEPARATION SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253910
作者单位	[Chen, Tao; Wang, Shuo; Shen, Cheng; Li, Hongmei; Wei, Yangfei; Yan, Shuping; Song, Zhibo; Li, Yulin] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China; [Chen, Tao; Yang, Xue; Li, Hongmei; Wei, Yangfei; Yan, Shuping; Song, Zhibo] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China; [Yang, Fengmei; Liu, Yarong; Hai, Ping] Qinghai Inst Food & Drug Control, Key Lab Qual Control Tradit Chinese Med Tibetan M, Natl Med Prod Adm, Xining 810000, Peoples R China
推荐引用方式 GB/T 7714	Chen, Tao,Yang, Xue,Wang, Shuo,et al. Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes[J]. 中国科学院西北生态环境资源研究院,2022,45(2).
APA	Chen, Tao.,Yang, Xue.,Wang, Shuo.,Shen, Cheng.,Li, Hongmei.,...&Li, Yulin.(2022).Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes.JOURNAL OF SEPARATION SCIENCE,45(2).
MLA	Chen, Tao,et al."Separation of five flavone glycosides including two groups with similar polarities from Dracocephalum tanguticum by a combination of three high-speed counter-current chromatography modes".JOURNAL OF SEPARATION SCIENCE 45.2(2022).