气候变化领域集成服务门户(CCPortal): De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.)

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DOI	10.3389/fpls.2021.625520
	De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.)
	Liu, Tao; Liu, Xiaoting; Zhou, Rangrang; Chen, Hong; Zhang, Huaigang; Zhang, Bo
通讯作者	Zhang, HG ; Zhang, B (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining, Peoples R China. ; Zhang, HG ; Zhang, B (通讯作者)，Qinghai Prov Key Lab Crop Mol Breeding, Xining, Peoples R China. ; Zhang, HG ; Zhang, B (通讯作者)，Univ Chinese Acad Sci, Beijing, Peoples R China.
发表日期	2021
ISSN	1664-462X
卷号	12
英文摘要	Selenium is an essential microelement for humans and animals. The specific processing technique of oats can maximize the preservation of its nutrients. In this study, to understand the genetic response of oats in a high-selenium environment, oats were treated with sodium selenate for 24 h, and transcriptome analysis was performed. A total of 211,485,930 clean reads composing 31.30 Gb of clean data were retained for four samples. After assembly, 186,035 unigenes with an average length of 727 bp were generated, and the N50 length was 1,149 bp. Compared with that in the control group, the expression of 7,226 unigenes in the treatment group was upregulated, and 2,618 unigenes were downregulated. Based on the sulfur assimilation pathway and selenocompound metabolic pathway, a total of 27 unigenes related to selenate metabolism were identified. Among them, the expression of both key genes APS (ATP sulfurylase) and APR (adenosine 5 '-phosphosulfate reductase) was upregulated more than 1,000-fold under selenate treatment, while that of CBL (cystathionine-beta-synthase) was upregulated 3.12-fold. Based on the transcriptome analysis, we suspect that the high-affinity sulfur transporter Sultr1;2 plays a key role in selenate uptake in oats. A preliminary regulatory mechanism explains the oat response to selenate treatment was ultimately proposed based on the transcriptome analysis and previous research.
关键词	MINERAL-COMPOSITION BIOFORTIFICATION OVEREXPRESSION VOLATILIZATION ANNOTATION EXPRESSION DATABASE GENOMES PLANTS CROPS
英文关键词	selenium; oats; transcriptome analysis; selenoproteins; selenium metabolism
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000668905000001
来源期刊	FRONTIERS IN PLANT SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253907
作者单位	[Liu, Tao; Zhou, Rangrang; Chen, Hong; Zhang, Huaigang; Zhang, Bo] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining, Peoples R China; [Liu, Tao; Zhou, Rangrang; Chen, Hong; Zhang, Huaigang; Zhang, Bo] Qinghai Prov Key Lab Crop Mol Breeding, Xining, Peoples R China; [Liu, Tao; Zhou, Rangrang; Chen, Hong; Zhang, Huaigang; Zhang, Bo] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Xiaoting] Xining 8 Jr High Sch, Xining, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tao,Liu, Xiaoting,Zhou, Rangrang,et al. De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.)[J]. 中国科学院西北生态环境资源研究院,2021,12.
APA	Liu, Tao,Liu, Xiaoting,Zhou, Rangrang,Chen, Hong,Zhang, Huaigang,&Zhang, Bo.(2021).De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.).FRONTIERS IN PLANT SCIENCE,12.
MLA	Liu, Tao,et al."De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.)".FRONTIERS IN PLANT SCIENCE 12(2021).