Climate Change Data Portal
| DOI | 10.1002/jssc.202001080 |
| Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay | |
| Liu, Chuang; Lei, Yuqing; Dang, Jun; Wang, Weidong; Zhang, Jing; Mei, Lijuan; Liu, Zenggen; Tao, Yanduo; Shao, Yun | |
| 通讯作者 | Tao, YD ; Shao, Y (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining, Qinghai, Peoples R China. |
| 发表日期 | 2021 |
| ISSN | 1615-9306 |
| EISSN | 1615-9314 |
| 起始页码 | 1345 |
| 结束页码 | 1352 |
| 卷号 | 44期号:7 |
| 英文摘要 | The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid chromatography under the guidance of an online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products. |
| 英文关键词 | free radical inhibitors; liquid chromatography; medium-pressure chromatography; Ribes himalense |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000616479000001 |
| 来源期刊 | JOURNAL OF SEPARATION SCIENCE
 |
| 来源机构 | 中国科学院西北生态环境资源研究院 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/253905 |
| 作者单位 | [Liu, Chuang; Dang, Jun; Wang, Weidong; Zhang, Jing; Mei, Lijuan; Liu, Zenggen; Tao, Yanduo; Shao, Yun] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining, Qinghai, Peoples R China; [Liu, Chuang; Lei, Yuqing; Wang, Weidong; Zhang, Jing] Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chuang,Lei, Yuqing,Dang, Jun,et al. Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay[J]. 中国科学院西北生态环境资源研究院,2021,44(7). |
| APA | Liu, Chuang.,Lei, Yuqing.,Dang, Jun.,Wang, Weidong.,Zhang, Jing.,...&Shao, Yun.(2021).Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay.JOURNAL OF SEPARATION SCIENCE,44(7). |
| MLA | Liu, Chuang,et al."Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay".JOURNAL OF SEPARATION SCIENCE 44.7(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
