气候变化领域集成服务门户(CCPortal): Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community

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DOI	10.3390/ijms23031694
	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community
	Tian, Yuan; Liu, Yang; Yue, Liang; Uwaremwe, Constantine; Zhao, Xia; Zhou, Qin; Wang, Yun; Wang, Ruoyu
通讯作者	Wang, RY (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Gansu Gaolan Field Sci Observat & Res Stn Agr Eco, Lanzhou 730000, Peoples R China. ; Wang, RY (通讯作者)，Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China.
发表日期	2022
EISSN	1422-0067
卷号	23 期号:3
英文摘要	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide. Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown. Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 x 10(5), 1.0 x 10(6), and 1.0 x 10(7) colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment. The results showed that LB + MS (1.0 x 10(5) CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 x 10(5) CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p <= 0.05). Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%. Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil. Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp. showed an effective suppression. Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability. Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential. Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
关键词	PLANT-GROWTH PROMOTING-RHIZOBACTERIA SECONDARY METABOLITES LIQUID-CHROMATOGRAPHY BIOLOGICAL-CONTROL YIELD NITROGEN QUALITY BIOFERTILIZER PHOSPHORUS
英文关键词	biocontrol; carbon sources; herbs; metabolome; nutrient utilization; plant-microbe interaction
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000757222700001
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253903
作者单位	[Tian, Yuan; Liu, Yang; Yue, Liang; Uwaremwe, Constantine; Zhao, Xia; Zhou, Qin; Wang, Yun; Wang, Ruoyu] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Gansu Gaolan Field Sci Observat & Res Stn Agr Eco, Lanzhou 730000, Peoples R China; [Tian, Yuan; Liu, Yang; Yue, Liang; Uwaremwe, Constantine; Zhao, Xia; Zhou, Qin; Wang, Ruoyu] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China; [Wang, Yun] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Desert & Desertificat, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Tian, Yuan,Liu, Yang,Yue, Liang,et al. Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community[J]. 中国科学院西北生态环境资源研究院,2022,23(3).
APA	Tian, Yuan.,Liu, Yang.,Yue, Liang.,Uwaremwe, Constantine.,Zhao, Xia.,...&Wang, Ruoyu.(2022).Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(3).
MLA	Tian, Yuan,et al."Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.3(2022).