气候变化领域集成服务门户(CCPortal): Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts

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DOI	10.3390/molecules26071946
	Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts
	Zhou, Wenna; Ouyang, Jian; Hu, Na; Li, Gang; Wang, Honglun
通讯作者	Wang, HL (通讯作者)，Northwest Inst Plateau Biol, CAS Key Lab Tibetan Med Res, Xining 810008, Peoples R China. ; Li, G (通讯作者)，Yantai Univ, Coll Life Sci, Ctr Mitochondria & Hlth Aging, Yantai 264005, Peoples R China. ; Wang, HL (通讯作者)，Chinese Acad Sci, Huzhou Plateau Biol Resource Ctr Innovat, Northwest Inst Plateau Biol, Huzhou 313000, Peoples R China.
发表日期	2021
EISSN	1420-3049
卷号	26 期号:7
英文摘要	Background: Doxorubicin (Dox) is one of the most frequently prescribed anti-cancer drugs. However, clinical application with Dox is limited due to its potentially fatal cumulative cardiotoxicity. N-p-coumaroyl-4-aminobutan-1-ol (alk-A), an organic amide alkaloid and hippophamide (alk-B), a rare pyridoindole alkaloid were successfully obtained by purification and separation of seabuckthorn seed residue in our previous research. This study was undertaken to investigate the protective effect of alk-A and alk-B against Dox-induced embryonic rat cardiac cells (H9c2 cells) apoptosis. Methods: H9c2 cells were treated with Dox (2.5 mu M) in the presence of alk-A and alk-B (10, 20, and 40 mu M) and incubated for 24 h. Results: It was shown that pretreatment of the H9c2 cells with alk-A and alk-B significantly reduced Dox-induced apoptosis. Alk-A and alk-B both inhibited reactive oxygen species (ROS) production and suppressed cleaved-caspase-3 protein expression and the activation of JNK (Jun N-terminal kinases), as well as increasing ATP levels, favoring mitochondrial mitofusin protein expression, and relieving damage to mitochondrial DNA. Conclusions: These results suggest that alk-A and alk-B can inhibit Dox-induced apoptosis in H9C2 cardiac muscle cells via inhibition of cell apoptosis and improvement of mitochondrial function, while alk-B showed more protection. Alk-B could be a potential candidate agent for protecting against cardiotoxicity in Dox-exposed patients.
英文关键词	alkaloid; doxorubicin; apoptosis; mitochondrial function; seabuckthorn
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000638729800001
来源期刊	MOLECULES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253891
作者单位	[Zhou, Wenna] Huzhou Univ, Dept Life Sci & Hlth, QiuZhen Coll, Huzhou 313000, Peoples R China; [Ouyang, Jian; Hu, Na; Wang, Honglun] Northwest Inst Plateau Biol, CAS Key Lab Tibetan Med Res, Xining 810008, Peoples R China; [Li, Gang] Yantai Univ, Coll Life Sci, Ctr Mitochondria & Hlth Aging, Yantai 264005, Peoples R China; [Ouyang, Jian; Wang, Honglun] Chinese Acad Sci, Huzhou Plateau Biol Resource Ctr Innovat, Northwest Inst Plateau Biol, Huzhou 313000, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Wenna,Ouyang, Jian,Hu, Na,et al. Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts[J]. 中国科学院西北生态环境资源研究院,2021,26(7).
APA	Zhou, Wenna,Ouyang, Jian,Hu, Na,Li, Gang,&Wang, Honglun.(2021).Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts.MOLECULES,26(7).
MLA	Zhou, Wenna,et al."Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts".MOLECULES 26.7(2021).