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DOI	10.3892/mmr.2021.11904
	Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells
	Jin, Youcai; Luan, Guangxiang; Li, Ji; Wang, Honglun; Wang, Zhenhua; Bai, Bo
通讯作者	Bai, B (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China. ; Wang, ZH (通讯作者)，Yantai Univ, Coll Life Sci, Ctr Mitochondria & Hlth Aging, 30 Qingquan Rd, Yantai 264005, Shandong, Peoples R China.
发表日期	2021
ISSN	1791-2997
EISSN	1791-3004
卷号	23 期号:4
英文摘要	Malignant tumors of the central nervous system (CNS) are among the types of cancer with the poorest prognosis and glioma is the commonest primary CNS tumor. A mitochondrial DNA (mtDNA)-depleted cell line C6 rho 0 was generated from C6 glioma cells after long-term exposure to ethidium bromide and 2 ',3 '-dideoxycytidine in order to determine the effect of mtDNA damage on cell proliferation and pathological changes in glioma cells. Single cell clones were isolated and identified after 42 days of incubation. Repopulated cybrids were formed when the clonal C6 rho 0 cells were fused with rat platelets and no difference was observed in their growth in a selective medium without uridine and pyruvate compared with the growth of the parent C6 cells. Disruption of mtDNA resulted in changes in mitochondrial morphology, decreased cell proliferation, reduced intracellular reactive oxygen species and intracellular ATP, along with decreased mtDNA and mitochondrial membrane potential in C6 rho 0 cells compared with the C6 cells. Taken together, C6 rho 0 cells without mtDNA were established for the first time and their characteristics were compared with parent cells. This C6 rho 0 cell line could be used to explore the contribution of mitochondrial dysfunction and mtDNA mutations in the pathogenesis of glioma.
英文关键词	C6 glioma; ρ 0; mitochondrial DNA; cytoplasmic hybrids; mitochondrial
语种	英语
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS类目	Oncology ; Medicine, Research & Experimental
WOS记录号	WOS:000620624100001
来源期刊	MOLECULAR MEDICINE REPORTS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253869
作者单位	[Jin, Youcai; Luan, Guangxiang; Wang, Honglun; Bai, Bo] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China; [Jin, Youcai] Qinghai Normal Univ, Coll Chem & Chem Engn, Xining 810008, Qinghai, Peoples R China; [Jin, Youcai; Luan, Guangxiang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Luan, Guangxiang; Wang, Honglun] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res Qinghai Prov, Xining 810008, Qinghai, Peoples R China; [Li, Ji; Wang, Honglun; Wang, Zhenhua] Yantai Univ, Coll Life Sci, Ctr Mitochondria & Hlth Aging, 30 Qingquan Rd, Yantai 264005, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Jin, Youcai,Luan, Guangxiang,Li, Ji,et al. Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells[J]. 中国科学院西北生态环境资源研究院,2021,23(4).
APA	Jin, Youcai,Luan, Guangxiang,Li, Ji,Wang, Honglun,Wang, Zhenhua,&Bai, Bo.(2021).Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells.MOLECULAR MEDICINE REPORTS,23(4).
MLA	Jin, Youcai,et al."Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6 rho 0 cells".MOLECULAR MEDICINE REPORTS 23.4(2021).