气候变化领域集成服务门户(CCPortal): Combined RNA-seq and Phenotype Analysis Reveals a Potential Molecular Mechanism of the Difference in Grain Size of Naked Barley From the Qinghai-Tibetan Plateau

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DOI	10.3389/fpls.2022.822607
	Combined RNA-seq and Phenotype Analysis Reveals a Potential Molecular Mechanism of the Difference in Grain Size of Naked Barley From the Qinghai-Tibetan Plateau
	Kong, Doudou; Xu, Jinqing; Wang, Lei; Wang, Handong; You, En; Li, Xiaolan; Chen, Tongrui; Shen, Yuhu
通讯作者	Xu, JQ ; Shen, YH (通讯作者)，Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Qinghai Prov Key Lab Crop Mol Breeding, Northwest Inst Plateau Biol, Xining, Peoples R China. ; Shen, YH (通讯作者)，Chinese Acad Sci, Lab Res & Utilizat Qinghai Tibetan Plateau Germpl, Northwest Inst Plateau Biol, Xining, Peoples R China. ; Shen, YH (通讯作者)，Chinese Acad Sci, Innovat Acad Seed Design, Xining, Peoples R China.
发表日期	2022
ISSN	1664-462X
卷号	13
英文摘要	To understand the molecular mechanism controlling the size of barley grains, a number of traits were analyzed and RNA-seq was conducted on grains of two barley materials with a significant difference in thousand-grain weight (TGW) after flowering. The trait dataset delineates the dynamic changes in grain size after flowering, and it provides an understanding of the source of the difference in TGW. By comparing the transcripts of barley grains at several stages after flowering, we identified the gene expression characteristics and significantly enriched pathways in each stage. At the early stage of grain development, genes involved in fatty acid metabolism, plant hormone signal transduction, and pathways involved in cytoskeleton formation were significantly upregulated. At the later stage of grain development, genes involved in starch synthesis, glucose metabolism, and other pathways were significantly upregulated. Further, we used weighted gene coexpression network analysis (WGCNA) and correlation analysis of trait datasets to identify the coexpressed gene modules significantly associated with traits, such as grain length (GL), grain width (GW), and dry weight (DW). After comparing the modules with the differentially expressed gene (DEG) set, 12 candidate genes were selected, and among these, four genes were homologous to genes that regulate grain size in rice and other plants. The combined analysis identified many potential key regulatory factors that may control barley grain size and yield potential, thus providing new insights into the molecular mechanism of barley grain size.
关键词	EARLY NODULIN ORGAN SIZE SEED SIZE RICE GENE EXPRESSION CONTRIBUTES PATHWAYS ENCODES NUMBER
英文关键词	barley; RNA-seq; grain size; DEGs; WGCNA
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000759861500001
来源期刊	FRONTIERS IN PLANT SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253850
作者单位	[Kong, Doudou; Xu, Jinqing; Wang, Lei; Wang, Handong; You, En; Li, Xiaolan; Chen, Tongrui; Shen, Yuhu] Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Qinghai Prov Key Lab Crop Mol Breeding, Northwest Inst Plateau Biol, Xining, Peoples R China; [Shen, Yuhu] Chinese Acad Sci, Lab Res & Utilizat Qinghai Tibetan Plateau Germpl, Northwest Inst Plateau Biol, Xining, Peoples R China; [Shen, Yuhu] Chinese Acad Sci, Innovat Acad Seed Design, Xining, Peoples R China
推荐引用方式 GB/T 7714	Kong, Doudou,Xu, Jinqing,Wang, Lei,et al. Combined RNA-seq and Phenotype Analysis Reveals a Potential Molecular Mechanism of the Difference in Grain Size of Naked Barley From the Qinghai-Tibetan Plateau[J]. 中国科学院西北生态环境资源研究院,2022,13.
APA	Kong, Doudou.,Xu, Jinqing.,Wang, Lei.,Wang, Handong.,You, En.,...&Shen, Yuhu.(2022).Combined RNA-seq and Phenotype Analysis Reveals a Potential Molecular Mechanism of the Difference in Grain Size of Naked Barley From the Qinghai-Tibetan Plateau.FRONTIERS IN PLANT SCIENCE,13.
MLA	Kong, Doudou,et al."Combined RNA-seq and Phenotype Analysis Reveals a Potential Molecular Mechanism of the Difference in Grain Size of Naked Barley From the Qinghai-Tibetan Plateau".FRONTIERS IN PLANT SCIENCE 13(2022).