气候变化领域集成服务门户(CCPortal): Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae)

CCPortal

DOI	10.3390/ani12020149
	Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae)
	Fu, Haibo; Li, Wenjing
通讯作者	Li, WJ (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China. ; Li, WJ (通讯作者)，Qinghai Prov Key Lab Anim Ecol Genom, Xining 810008, Peoples R China.
发表日期	2022
ISSN	2076-2615
卷号	12 期号:2
英文摘要	Simple Summary Plateau pika produces hard and soft feces with different morphology, component and microbial structure. Hard feces had more abundant Firmicutes, while soft feces had more abundant Akkermansia. The differences of microbial communities between hard and soft feces were mainly driven by core microbomes. Soft feces had a comprehensive advances in predict functional pathways compared to hard feces, these strengthened functional pathways were closely associated with metabolism of energy, vitamins, and amino acid. Our study preliminarily explored the differences in microbial structure and function between hard and soft feces, provided a foundation for future systematic explorations of the cecotrophy. The division of hard and soft feces is an effective digestion strategy in the order Lagomorpha. Although previous studies have reported that hard and soft feces differ in morphology and component, the discrepancy in the microbiome remains unclear. This study explored the microbiomes of hard and soft feces in plateau pikas by sequencing the V3 and V4 regions of 16S rDNA. We found that hard feces harbored higher Firmicutes, while soft feces harbored higher Akkermansia. Increased rare bacterial taxa were observed in hard feces compared with soft feces. Moreover, hard and soft feces displayed a greater difference in terms of core operational taxonomy units (OTUs) compared to the total OTUs. The soft feces showed enhancements in all predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) functions, indicating an advancing microbial metabolism compared to hard feces. The significantly upregulated pathways in soft feces were mainly enriched in metabolism of energy and carbohydrate, glycan biosynthesis, cofactors and vitamins, and amino acids-all of which are associated with increased contents of microbial proteins, vitamins, and short-chain fatty acids. Our study reports, for the first time, the differential microbiomes between hard and soft feces of pikas and provides direction for the future studies on cecotrophy.
关键词	COPROPHAGY RABBIT DIVERSITY MOTILITY DIGESTA COLON DIET
英文关键词	gut microbiota; plateau pika; soft feces; hard feces; cecotrophy
语种	英语
WOS研究方向	Agriculture ; Veterinary Sciences ; Zoology
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences ; Zoology
WOS记录号	WOS:000746237200001
来源期刊	ANIMALS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253803
作者单位	[Fu, Haibo; Li, Wenjing] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China; [Fu, Haibo; Li, Wenjing] Qinghai Prov Key Lab Anim Ecol Genom, Xining 810008, Peoples R China; [Fu, Haibo] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fu, Haibo,Li, Wenjing. Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae)[J]. 中国科学院西北生态环境资源研究院,2022,12(2).
APA	Fu, Haibo,&Li, Wenjing.(2022).Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae).ANIMALS,12(2).
MLA	Fu, Haibo,et al."Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae)".ANIMALS 12.2(2022).