气候变化领域集成服务门户(CCPortal): Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana

CCPortal

DOI	10.1134/S1021443721060029
	Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana
	Chen, C.; Liu, Y.; Liu, M.
通讯作者	Chen, C (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Lanzhou 730000, Peoples R China.
发表日期	2021
ISSN	1021-4437
EISSN	1608-3407
起始页码	1079
结束页码	1086
卷号	68 期号:6
英文摘要	In egy1 (ethylene-dependent gravitropism-deficient and yellow-green 1) mutants of Arabidopsis thaliana (L.) Heynh., there is reduced granal thylakoids, smaller size and less number of plastids in endodermal cells, suggesting that EGY1 protein is required for chloroplast development. Chloroplast is the apparatus of photosynthesis, how do egy1 mutants affect photosynthesis? Here we have further characterized the function of EGY1 protein in photosynthesis. The chlorophyll content and F-v/F-m (maximum photochemical efficiency of PSII) decreased much more rapidly with increasing leaf age in egy1 mutants than in wild-type (WT) plants of A.thaliana. The results of blue native-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (BN-SDS-PAGE) suggested that EGY1 was associated with photosystem II (PSII) supercomplexes. The spectroscopic and immunoblot analyses further showed that accelerated reductions in the abundance of D1, D2 and light harvesting complex II (LHCII) occurred with increasing leaf age in egy1 mutants. Moreover, PSII proteins (D1 and LHCII) were less stable in egy1 mutants than in WT plants under high light condition. Pull-down assays showed that EGY1 was colocalized with D2 protein. These results showed that loss-function of EGY1 results in accelerated reduction of PSII and in decreased stability of PSII, suggesting that defection of EGY1 results in impaired photosynthesis through breaking down the interaction of EGY1 with PSII in A. thaliana.
关键词	LIGHT CHLOROPLASTS DEGRADATION COMPLEXES ENCODES
英文关键词	Arabidopsis thaliana; egy1 mutant; photosynthetic complex; photosystem II content; stability; thylakoid membrane
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000713613100009
来源期刊	RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253795
作者单位	[Chen, C.; Liu, Y.; Liu, M.] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Stress Physiol & Ecol Cold & Arid Reg, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Chen, C.,Liu, Y.,Liu, M.. Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana[J]. 中国科学院西北生态环境资源研究院,2021,68(6).
APA	Chen, C.,Liu, Y.,&Liu, M..(2021).Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana.RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,68(6).
MLA	Chen, C.,et al."Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana".RUSSIAN JOURNAL OF PLANT PHYSIOLOGY 68.6(2021).