气候变化领域集成服务门户(CCPortal): Differential Expression of Metabolism-Related Genes in Plateau Pika (Ochotona curzoniae) at Different Altitudes on the Qinghai-Tibet Plateau

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DOI	10.3389/fgene.2021.784811
	Differential Expression of Metabolism-Related Genes in Plateau Pika (Ochotona curzoniae) at Different Altitudes on the Qinghai-Tibet Plateau
	Zhu, Hongjuan; Zhong, Liang; Li, Jing; Wang, Suqin; Qu, Jiapeng
通讯作者	Qu, JP (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China. ; Qu, JP (通讯作者)，Qinghai Prov Key Lab Anim Ecol Genom, Xining, Peoples R China.
发表日期	2022
EISSN	1664-8021
卷号	12
英文摘要	According to life history theory, animals living in extreme environments have evolved specific behavioral and physiological strategies for survival. However, the genetic mechanisms underpinning these strategies are unclear. As the highest geographical unit on Earth, the Qinghai-Tibet Plateau is characterized by an extreme environment and climate. During long-term evolutionary processes, animals that inhabit the plateau have evolved specialized morphological and physiological traits. The plateau pika (Ochotona curzoniae), one of the native small mammals that evolved on the Qinghai-Tibet Plateau, has adapted well to this cold and hypoxic environment. To explore the genetic mechanisms underlying the physiological adaptations of plateau pika to extremely cold ambient temperatures, we measured the differences in resting metabolic rate (RMR) and metabolism-related gene expression in individuals inhabiting three distinct altitudes (i.e., 3,321, 3,663, and 4,194 m). Results showed that the body mass and RMR of plateau pika at high- and medium-altitudes were significantly higher than those at the low-altitude. The expression levels of peroxisome proliferator-activated receptor alpha (ppar alpha), peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (pgc-1 alpha), and the PR domain-containing 16 (PRDM16) in white (WAT) and brown (BAT) adipose tissues of plateau pika from high- and medium-altitudes were significantly higher than in pika from the low-altitude region. The enhanced expression levels of pgc-1 alpha and ppar alpha genes in the WAT of pika at high-altitude showed that WAT underwent browning and increased thermogenic properties. An increase in the expression of uncoupling protein 1 (UCP1) in the BAT of pika at high altitude indicated that BAT increased their thermogenic properties. The gene expression levels of ppar alpha and pgc-1 alpha in skeletal muscles were significantly higher in high-altitude pika. Simultaneously, the expression of the sarcolipin (SLN) gene in skeletal muscles significantly increased in high-altitude pika. Our results suggest that plateau pika adapted to an extremely cold environment via browning WAT, thereby activating BAT and enhancing SLN expression to increase non-shivering thermogenesis. This study demonstrates that plateau pika can increase thermogenic gene expression and energy metabolism to adapt to the extreme environments on the plateau.
关键词	BROWN ADIPOSE-TISSUE BODY-MASS REGULATION NONSHIVERING THERMOGENESIS ADAPTIVE THERMOGENESIS PHENOTYPIC FLEXIBILITY HEMOGLOBIN-FUNCTION ENERGY-METABOLISM TUPAIA-BELANGERI SMALL MAMMALS WILD-TYPE
英文关键词	plateau pika; thermogenic capacity; energy metabolism; gene expression; altitude
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000750097600001
来源期刊	FRONTIERS IN GENETICS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253781
作者单位	[Zhu, Hongjuan; Zhong, Liang; Li, Jing; Wang, Suqin; Qu, Jiapeng] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China; [Zhu, Hongjuan; Li, Jing; Wang, Suqin] Univ Chinese Acad Sci, Beijing, Peoples R China; [Zhong, Liang; Qu, Jiapeng] Qinghai Prov Key Lab Anim Ecol Genom, Xining, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Hongjuan,Zhong, Liang,Li, Jing,et al. Differential Expression of Metabolism-Related Genes in Plateau Pika (Ochotona curzoniae) at Different Altitudes on the Qinghai-Tibet Plateau[J]. 中国科学院西北生态环境资源研究院,2022,12.
APA	Zhu, Hongjuan,Zhong, Liang,Li, Jing,Wang, Suqin,&Qu, Jiapeng.(2022).Differential Expression of Metabolism-Related Genes in Plateau Pika (Ochotona curzoniae) at Different Altitudes on the Qinghai-Tibet Plateau.FRONTIERS IN GENETICS,12.
MLA	Zhu, Hongjuan,et al."Differential Expression of Metabolism-Related Genes in Plateau Pika (Ochotona curzoniae) at Different Altitudes on the Qinghai-Tibet Plateau".FRONTIERS IN GENETICS 12(2022).