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DOI	10.1002/jssc.202100544
	The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example
	Zou, Denglang; Cui, Yunbin; Li, Si; Sang, Duocheng; Liu, Weimeng; Zhao, Tianshu; Gu, Xueli; Chen, Tao; Li, Yulin
通讯作者	Zou, DL (通讯作者)，Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Sch Life Sci, Key Lab Biodivers Format Mech & Comprehens Utiliz, Xining, Peoples R China. ; Chen, T (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China.
发表日期	2021
ISSN	1615-9306
EISSN	1615-9314
起始页码	4368
结束页码	4375
卷号	44 期号:24
英文摘要	Biosynthesis is a promising way to manufacture desired products, however, the purification of its final products is a tough work due to the huge amount of reaction matrix. Liquid stationary phase of high-speed counter-current chromatography could easily avoid the commonly disadvantages that occurred in traditional column chromatography in the field of biosynthesized products purification. This characteristic makes high-speed counter-current chromatography particularly applicable for final products separation in biosynthesis. In this study, the glycosylation products of Silybin B by one-pot glycosylation were successfully purified by high-speed counter-current chromatography to show the applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products. An optimized n-hexane/ethyl acetate/methanol/water (2:5:2:3, v/v/v/v) system was applied in this study. As a result, four Silybin B glycosylation products, including 7 mg of Silybin B-5-O-beta-D-glucoside (SG-1), 12 mg of Silybin B-3-O-beta-D-glucoside (SG-2), 10 mg of Silybin B-7-O-beta-D-glucoside (SG-3), and 24 mg of Silybin B-20-O-beta-D-glucoside (SG-4), were simultaneously separated from 200 mg of glycosylation crude products, with the purity of 89.3, 95.2, 96.4, and 97.5%, respectively. Their structures were identified by spectroscopic analysis.
关键词	EFFICIENT STRATEGY ESCHERICHIA-COLI GLYCOSYLTRANSFERASE BUFADIENOLIDE EXTRACTION SILYBIN
英文关键词	biosynthesis; glycosylation; high-speed counter-current chromatography; Silybin B
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000713457400001
来源期刊	JOURNAL OF SEPARATION SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253764
作者单位	[Zou, Denglang; Cui, Yunbin; Li, Si; Sang, Duocheng; Liu, Weimeng; Zhao, Tianshu; Gu, Xueli] Qinghai Normal Univ, Acad Plateau Sci & Sustainabil, Sch Life Sci, Key Lab Biodivers Format Mech & Comprehens Utiliz, Xining, Peoples R China; [Chen, Tao; Li, Yulin] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China
推荐引用方式 GB/T 7714	Zou, Denglang,Cui, Yunbin,Li, Si,et al. The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example[J]. 中国科学院西北生态环境资源研究院,2021,44(24).
APA	Zou, Denglang.,Cui, Yunbin.,Li, Si.,Sang, Duocheng.,Liu, Weimeng.,...&Li, Yulin.(2021).The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example.JOURNAL OF SEPARATION SCIENCE,44(24).
MLA	Zou, Denglang,et al."The applicability of high-speed counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example".JOURNAL OF SEPARATION SCIENCE 44.24(2021).