气候变化领域集成服务门户(CCPortal): Development of immunocapture reverse transcription loop-mediated isothermal amplification assays to detect both lily symptomless virus and cucumber mosaic virus

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DOI	10.1111/aab.12716
	Development of immunocapture reverse transcription loop-mediated isothermal amplification assays to detect both lily symptomless virus and cucumber mosaic virus
	Zhang, Yubao; Xie, Zhongkui; Wang, Yajun; Wang, Ruoyu; Guo, Zhihong; Qiu, Yang; He, Yuhui
通讯作者	Xie, ZK (通讯作者)，Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Lanzhou 730000, Peoples R China.
发表日期	2022
ISSN	0003-4746
EISSN	1744-7348
起始页码	132
结束页码	139
卷号	180 期号:1
英文摘要	Lily symptomless virus (LSV) and cucumber mosaic virus (CMV) are two of the most prevalent viruses that infect lily plants around the world. Infections of the edible Lanzhou lily (Lilium davidii var. unicolor) by these viruses seriously decrease crop yield in northwestern China. Development of reliable, practical and sensitive diagnostic tools is essential for surveillance and management of the viruses. Here we describe the development of immunocapture (IC) reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays for detecting both viruses. This approach captures viral antigens using antibodies (anti-rabbit IgG) against recombinant LSV or CMV coat proteins and then detects them by means of RT-LAMP, without needing to isolate RNA. Optimal conditions for both LAMP reactions were 60 degrees C for 60 min. The detection limit of both assays was 100 times more sensitive than the IC-RT-polymerase chain reaction assay. The IC-RT-LAMP assay results are also visible to the naked eye using SYBR Green dye. This is the first study to report the detection of LSV and CMV in lily using an IC-RT-LAMP assay. We believe that this convenient and sensitive method has excellent potential for enhanced diagnosis of field-collected samples, especially where laboratory facilities and technical resources are limited or absent.
关键词	RAPID DETECTION MOTTLE-VIRUS SENSITIVE DETECTION RT-PCR LAMP
英文关键词	cucumber mosaic virus; diagnosis technology; IC-RT-LAMP assay; lily symptomless virus
语种	英语
WOS研究方向	Agriculture
WOS类目	Agriculture, Multidisciplinary
WOS记录号	WOS:000675548700001
来源期刊	ANNALS OF APPLIED BIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253654
作者单位	[Zhang, Yubao; Xie, Zhongkui; Wang, Yajun; Wang, Ruoyu; Guo, Zhihong; Qiu, Yang; He, Yuhui] Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yubao,Xie, Zhongkui,Wang, Yajun,et al. Development of immunocapture reverse transcription loop-mediated isothermal amplification assays to detect both lily symptomless virus and cucumber mosaic virus[J]. 中国科学院西北生态环境资源研究院,2022,180(1).
APA	Zhang, Yubao.,Xie, Zhongkui.,Wang, Yajun.,Wang, Ruoyu.,Guo, Zhihong.,...&He, Yuhui.(2022).Development of immunocapture reverse transcription loop-mediated isothermal amplification assays to detect both lily symptomless virus and cucumber mosaic virus.ANNALS OF APPLIED BIOLOGY,180(1).
MLA	Zhang, Yubao,et al."Development of immunocapture reverse transcription loop-mediated isothermal amplification assays to detect both lily symptomless virus and cucumber mosaic virus".ANNALS OF APPLIED BIOLOGY 180.1(2022).