气候变化领域集成服务门户(CCPortal): Gastrointestinal Tract and Dietary Fiber Driven Alterations of Gut Microbiota and Metabolites in Durco x Bamei Crossbred Pigs

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DOI	10.3389/fnut.2021.806646
	Gastrointestinal Tract and Dietary Fiber Driven Alterations of Gut Microbiota and Metabolites in Durco x Bamei Crossbred Pigs
	Wu, Guofang; Tang, Xianjiang; Fan, Chao; Wang, Lei; Shen, Wenjuan; Ren, Shi'en; Zhang, Liangzhi; Zhang, Yanming
通讯作者	Zhang, LZ ; Zhang, YM (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China. ; Zhang, LZ ; Zhang, YM (通讯作者)，Northwest Inst Plateau Biol, Qinghai Prov Key Lab Anim Ecol Gen, Xining, Peoples R China.
发表日期	2022
ISSN	2296-861X
卷号	8
英文摘要	Gastrointestinal tract and dietary fiber (DF) are known to influence gut microbiome composition. However, the combined effect of gut segment and long-term intake of a high fiber diet on pig gut microbiota and metabolite profiles is unclear. Here, we applied 16S rRNA gene sequencing and untargeted metabolomics to investigate the effect of broad bean silage on the composition and metabolites of the cecal and jejunal microbiome in Durco x Bamei crossbred pigs. Twenty-four pigs were allotted to four graded levels of DF chow, and the content of jejunum and cecum were collected. Our results demonstrated that cecum possessed higher alpha-diversity and abundance of Bacteroidetes, unidentified Ruminococcaceae compared to jejunum, while jejunum possessed higher abundance of Lactobacillus, Streptococcus. DF intake significantly altered diversity of the bacterial community. The abundance of Bacteroidetes and Turicibacter increased with the increase of DF in cecum and jejunum respectively. Higher concentrations of amino acids and conjugated bile acids were detected in the jejunum, whereas free bile acids and fatty acids were enriched in the cecum. The concentrations of fatty acids, carbohydrate metabolites, organic acids, 2-oxoadipic acid, and succinate in cecum were higher in the high DF groups. Overall, the results indicate that the composition of bacteria and the microbiota metabolites were distinct in different gut segments. DF had a significant influence on the bacterial composition and structure in the cecum and jejunum, and that the cecal metabolites may further affect host health, growth, and slaughter performance.
关键词	GROWTH-PERFORMANCE FERMENTABLE FIBER BILE-ACIDS IMPACT INGREDIENTS DIGESTIBILITY COMMUNITY NITROGEN JEJUNUM HEALTH
英文关键词	dietary fiber; gut bacteria; metabolites; gut segment; Durco x Bamei crossbred pig
语种	英语
WOS研究方向	Nutrition & Dietetics
WOS类目	Nutrition & Dietetics
WOS记录号	WOS:000760545000001
来源期刊	FRONTIERS IN NUTRITION
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253625
作者单位	[Wu, Guofang; Tang, Xianjiang; Fan, Chao; Ren, Shi'en; Zhang, Liangzhi; Zhang, Yanming] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China; [Wu, Guofang; Wang, Lei; Shen, Wenjuan] Qinghai Univ, Qinghai Acad Anim & Vet Med, Plateau Livestock Genet Resources Protect & Innov, Xining, Peoples R China; [Tang, Xianjiang; Fan, Chao; Ren, Shi'en; Zhang, Liangzhi; Zhang, Yanming] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Anim Ecol Gen, Xining, Peoples R China; [Tang, Xianjiang; Fan, Chao; Ren, Shi'en] Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Guofang,Tang, Xianjiang,Fan, Chao,et al. Gastrointestinal Tract and Dietary Fiber Driven Alterations of Gut Microbiota and Metabolites in Durco x Bamei Crossbred Pigs[J]. 中国科学院西北生态环境资源研究院,2022,8.
APA	Wu, Guofang.,Tang, Xianjiang.,Fan, Chao.,Wang, Lei.,Shen, Wenjuan.,...&Zhang, Yanming.(2022).Gastrointestinal Tract and Dietary Fiber Driven Alterations of Gut Microbiota and Metabolites in Durco x Bamei Crossbred Pigs.FRONTIERS IN NUTRITION,8.
MLA	Wu, Guofang,et al."Gastrointestinal Tract and Dietary Fiber Driven Alterations of Gut Microbiota and Metabolites in Durco x Bamei Crossbred Pigs".FRONTIERS IN NUTRITION 8(2022).