气候变化领域集成服务门户(CCPortal): Effect of Dietary Protein Levels on Dynamic Changes and Interactions of Ruminal Microbiota and Metabolites in Yaks on the Qinghai-Tibetan Plateau

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DOI	10.3389/fmicb.2021.684340
	Effect of Dietary Protein Levels on Dynamic Changes and Interactions of Ruminal Microbiota and Metabolites in Yaks on the Qinghai-Tibetan Plateau
	Zhang, XiaoLing; Xu, TianWei; Wang, XunGang; Geng, YuanYue; Zhao, Na; Hu, LinYong; Liu, HongJin; Kang, ShengPing; Xu, ShiXiao
通讯作者	Xu, SX (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China.
发表日期	2021
EISSN	1664-302X
卷号	12
英文摘要	To improve performance and optimize rumen function in yaks (Bos grunniens), further knowledge on the appropriate dietary protein levels for ruminal microbiota and the metabolite profiles of yaks in feedlot feeding is necessary. Current understanding of dietary protein requirements, ruminal microbiota, and metabolites is limited. In this study, yaks were fed a low-protein diet (L; 9.64%), middle low-protein diet (ML; 11.25%), middle high-protein diet (MH; 12.48%), or a high-protein diet (H; 13.87%), and the effects of those diets on changes and interactions in ruminal microbiota and metabolites were investigated. Twenty-four female yaks were selected, and the effects on ruminal microbiota and metabolites were investigated using 16s rRNA gene sequencing and gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). Diets containing different protein levels changed the composition of the rumen bacterial community, the H group significantly reduced the diversity of ruminal microbiota (p < 0.05), and the number of shared amplicon sequence variants (ASVs) between the H group and the other three groups was lower, suggesting that the ruminal microbiota community fluctuated more with a high-protein diet. In rumen, Bacteroidetes, Firmicutes, and Proteobacteria were the most abundant bacteria at the phylum level, and Bacteroidetes was significantly less abundant in the MH group than in the L and ML groups (p < 0.05). Prevotella_1, Rikenellaceae_RC9_gut_group, and Christensenellaceae_R-7_group had the highest abundance at the genus level. Prevotellaceae was enriched in the low-protein groups, whereas Bacteroidales_BS11_gut_group was enriched in the high-protein groups. Rumen metabolite concentrations and metabolic patterns were altered by dietary protein levels: organic acid metabolites, antioxidant-related metabolites, and some plant-derived metabolites showed variation between the groups. Enrichment analysis revealed that significant changes were concentrated in six pathways, including the citrate cycle (TCA cycle), glyoxylate and dicarboxylate metabolism, and butanoate metabolism. Network analysis showed promotion or restraint relationships between different rumen microbiota and metabolites. Overall, the rumen function was higher in the MH group. This study provides a reference for appropriate dietary protein levels and improves understanding of rumen microbes and metabolites.
关键词	RUMEN BACTERIAL COMMUNITY GUT MICROBIOTA PHYTANIC ACID RICE STRAW L-DOPA QUANTIFICATION METABOLOMICS PREVOTELLA DIVERSITY
英文关键词	yak; dietary protein; rumen; microbiota; metabolites
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000687645200001
来源期刊	FRONTIERS IN MICROBIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253606
作者单位	[Zhang, XiaoLing; Xu, TianWei; Wang, XunGang; Geng, YuanYue; Zhao, Na; Hu, LinYong; Liu, HongJin; Kang, ShengPing; Xu, ShiXiao] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China; [Zhang, XiaoLing; Wang, XunGang; Geng, YuanYue; Liu, HongJin; Kang, ShengPing] Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, XiaoLing,Xu, TianWei,Wang, XunGang,et al. Effect of Dietary Protein Levels on Dynamic Changes and Interactions of Ruminal Microbiota and Metabolites in Yaks on the Qinghai-Tibetan Plateau[J]. 中国科学院西北生态环境资源研究院,2021,12.
APA	Zhang, XiaoLing.,Xu, TianWei.,Wang, XunGang.,Geng, YuanYue.,Zhao, Na.,...&Xu, ShiXiao.(2021).Effect of Dietary Protein Levels on Dynamic Changes and Interactions of Ruminal Microbiota and Metabolites in Yaks on the Qinghai-Tibetan Plateau.FRONTIERS IN MICROBIOLOGY,12.
MLA	Zhang, XiaoLing,et al."Effect of Dietary Protein Levels on Dynamic Changes and Interactions of Ruminal Microbiota and Metabolites in Yaks on the Qinghai-Tibetan Plateau".FRONTIERS IN MICROBIOLOGY 12(2021).