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DOI	10.1038/s41467-021-21338-6
	Towards plant resistance to viruses using protein-only RNase P
	Gobert A.; Quan Y.; Arrivé M.; Waltz F.; Da Silva N.; Jomat L.; Cohen M.; Jupin I.; Giegé P.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Plant viruses cause massive crop yield loss worldwide. Most plant viruses are RNA viruses, many of which contain a functional tRNA-like structure. RNase P has the enzymatic activity to catalyze the 5′ maturation of precursor tRNAs. It is also able to cleave tRNA-like structures. However, RNase P enzymes only accumulate in the nucleus, mitochondria, and chloroplasts rather than cytosol where virus replication takes place. Here, we report a biotechnology strategy based on the re-localization of plant protein-only RNase P to the cytosol (CytoRP) to target plant viruses tRNA-like structures and thus hamper virus replication. We demonstrate the cytosol localization of protein-only RNase P in Arabidopsis protoplasts. In addition, we provide in vitro evidences for CytoRP to cleave turnip yellow mosaic virus and oilseed rape mosaic virus. However, we observe varied in vivo results. The possible reasons have been discussed. Overall, the results provided here show the potential of using CytoRP for combating some plant viral diseases. © 2021, The Author(s).
语种	英语
scopus关键词	genomic RNA; plant protein; ribonuclease P; transfer RNA; virus RNA; ribonuclease P; RNA precursor; transfer RNA; biotechnology; cell organelle; crop yield; enzyme; enzyme activity; herb; protein; RNA; viral disease; virus; affect; Arabidopsis thaliana; Article; cell nucleus; chloroplast; controlled study; cytosol; environmental impact; enzyme activity; enzyme structure; harvest; in vitro study; mitochondrion; nonhuman; nuclear localization signal; plant virus; protein expression; protein localization; protein purification; rapeseed; RNA cleavage; RNA isolation; RNA structure; transgenic plant; Turnip yellow mosaic virus; virus replication; virus resistance; Arabidopsis; chemistry; disease resistance; genetics; metabolism; physiology; plant virus; protoplast; Arabidopsis; Chinese Rape Mosaic Virus; RNA viruses; Turnip yellow mosaic virus; Arabidopsis; Chloroplasts; Disease Resistance; Mosaic Viruses; Plant Viruses; Protoplasts; Ribonuclease P; RNA Precursors; RNA, Transfer
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251511
作者单位	Institut de biologie moléculaire des plantes, UPR2357 du CNRS, Université de Strasbourg, Strasbourg, France; Institut Jacques Monod, Laboratory of Molecular Virology, UMR7592 CNRS, Université de Paris, Paris, France
推荐引用方式 GB/T 7714	Gobert A.,Quan Y.,Arrivé M.,et al. Towards plant resistance to viruses using protein-only RNase P[J],2021,12(1).
APA	Gobert A..,Quan Y..,Arrivé M..,Waltz F..,Da Silva N..,...&Giegé P..(2021).Towards plant resistance to viruses using protein-only RNase P.Nature Communications,12(1).
MLA	Gobert A.,et al."Towards plant resistance to viruses using protein-only RNase P".Nature Communications 12.1(2021).