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DOI	10.1038/s41467-021-22176-2
	Bacterial cyclic diguanylate signaling networks sense temperature
	Almblad H.; Randall T.E.; Liu F.; Leblanc K.; Groves R.A.; Kittichotirat W.; Winsor G.L.; Fournier N.; Au E.; Groizeleau J.; Rich J.D.; Lou Y.; Granton E.; Jennings L.K.; Singletary L.A.; Winstone T.M.L.; Good N.M.; Bumgarner R.E.; Hynes M.F.; Singh M.; Stietz M.S.; Brinkman F.S.L.; Kumar A.; Brassinga A.K.C.; Parsek M.R.; Tseng B.S.; Lewis I.A.; Yipp B.G.; MacCallum J.L.; Harrison J.J.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Many bacteria use the second messenger cyclic diguanylate (c-di-GMP) to control motility, biofilm production and virulence. Here, we identify a thermosensory diguanylate cyclase (TdcA) that modulates temperature-dependent motility, biofilm development and virulence in the opportunistic pathogen Pseudomonas aeruginosa. TdcA synthesizes c-di-GMP with catalytic rates that increase more than a hundred-fold over a ten-degree Celsius change. Analyses using protein chimeras indicate that heat-sensing is mediated by a thermosensitive Per-Arnt-SIM (PAS) domain. TdcA homologs are widespread in sequence databases, and a distantly related, heterologously expressed homolog from the Betaproteobacteria order Gallionellales also displayed thermosensitive diguanylate cyclase activity. We propose, therefore, that thermotransduction is a conserved function of c-di-GMP signaling networks, and that thermosensitive catalysis of a second messenger constitutes a mechanism for thermal sensing in bacteria. © 2021, The Author(s).
语种	英语
scopus关键词	chimeric protein; cyclic diguanylate; diguanylate cyclase; guanosine phosphate; guanylate cyclase; unclassified drug; bacterial protein; bis(3',5')-cyclic diguanylic acid; cyclic GMP; diguanylate cyclase; Escherichia coli protein; lyase; bacterium; biofilm; catalysis; catalyst; database; enzyme; enzyme activity; gene expression; Article; bacterial virulence; Betaproteobacteria; biofilm; catalysis; comparative genomics; controlled study; enzyme activity; Gallionellales; genetic conservation; genetic linkage; heat; nonhuman; nucleotide metabolism; Pseudomonas aeruginosa; second messenger; sequence database; signal transduction; temperature dependence; algorithm; gene expression regulation; genetics; growth, development and aging; liquid chromatography; mass spectrometry; metabolism; physiology; Pseudomonas aeruginosa; second messenger; signal transduction; temperature; Bacteria (microorganisms); Betaproteobacteria; Pseudomonas aeruginosa; Algorithms; Bacterial Proteins; Biofilms; Chromatography, Liquid; Cyclic GMP; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Mass Spectrometry; Phosphorus-Oxygen Lyases; Pseudomonas aeruginosa; Second Messenger Systems; Signal Transduction; Temperature
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251483
作者单位	Department of Biological Sciences, University of Calgary, Calgary, AB, Canada; Systems Biology and Bioinformatics Research Group, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada; Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Department of Microbiology, University of Washington, Seattle, WA, United States; Department of Chemistry, University of Calgary, Calgary, AB, Canada; Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada; School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, United States
推荐引用方式 GB/T 7714	Almblad H.,Randall T.E.,Liu F.,et al. Bacterial cyclic diguanylate signaling networks sense temperature[J],2021,12(1).
APA	Almblad H..,Randall T.E..,Liu F..,Leblanc K..,Groves R.A..,...&Harrison J.J..(2021).Bacterial cyclic diguanylate signaling networks sense temperature.Nature Communications,12(1).
MLA	Almblad H.,et al."Bacterial cyclic diguanylate signaling networks sense temperature".Nature Communications 12.1(2021).